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Accurate micro-computed tomography imaging of pore spaces in collagen-based scaffold

Zidek, Jan
Author Zidek, Jan CEITEC-Central European Institute of Technology, Brno University of Technology, Purkynova 123, 61200, Brno, Czech Republic. jan.zidek@ceitec.vutbr.cz
Vojtova, Lucy
Author Vojtova, Lucy CEITEC-Central European Institute of Technology, Brno University of Technology, Purkynova 123, 61200, Brno, Czech Republic SCITEG, a.s., Brno, Czech Republic
Abdel-Mohsen, A M
Author Abdel-Mohsen, A M CEITEC-Central European Institute of Technology, Brno University of Technology, Purkynova 123, 61200, Brno, Czech Republic Textile Research Division, National Research Centre, El-Buhouth St, P.O. Box 12311, Cairo, Egypt
Chmelik, Jiri
Author Chmelik, Jiri Institute of Biomedical Engineering, FEEC, Brno University of Technology, Technicka 12, 61600, Brno, Czech Republic
Zikmund, Tomas
Author Zikmund, Tomas CEITEC-Central European Institute of Technology, Brno University of Technology, Purkynova 123, 61200, Brno, Czech Republic
Brtnikova, Jana
Author Brtnikova, Jana CEITEC-Central European Institute of Technology, Brno University of Technology, Purkynova 123, 61200, Brno, Czech Republic
Jakubicek, Roman
Author Jakubicek, Roman Institute of Biomedical Engineering, FEEC, Brno University of Technology, Technicka 12, 61600, Brno, Czech Republic
Zubal, Lukas
Author Zubal, Lukas CEITEC-Central European Institute of Technology, Brno University of Technology, Purkynova 123, 61200, Brno, Czech Republic
Jan, Jiri
Author Jan, Jiri Institute of Biomedical Engineering, FEEC, Brno University of Technology, Technicka 12, 61600, Brno, Czech Republic
Kaiser, Jozef
Author Kaiser, Jozef CEITEC-Central European Institute of Technology, Brno University of Technology, Purkynova 123, 61200, Brno, Czech Republic

Journal of materials science. Materials in medicine. 2016 Jun ; 27 (6) : 110. [epub] 20160506

J Mater Sci Mater Med
ISSN 1573-4838 | 0957-4530
Source

Language English Country United States Media print-electronic

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid27153826

PubMed 27153826
DOI 10.1007/s10856-016-5717-2
PII: 10.1007/s10856-016-5717-2
Knihovny.cz E-resources

In this work we have used X-ray micro-computed tomography (μCT) as a method to observe the morphology of 3D porous pure collagen and collagen-composite scaffolds useful in tissue engineering. Two aspects of visualizations were taken into consideration: improvement of the scan and investigation of its sensitivity to the scan parameters. Due to the low material density some parts of collagen scaffolds are invisible in a μCT scan. Therefore, here we present different contrast agents, which increase the contrast of the scanned biopolymeric sample for μCT visualization. The increase of contrast of collagenous scaffolds was performed with ceramic hydroxyapatite microparticles (HAp), silver ions (Ag(+)) and silver nanoparticles (Ag-NPs). Since a relatively small change in imaging parameters (e.g. in 3D volume rendering, threshold value and μCT acquisition conditions) leads to a completely different visualized pattern, we have optimized these parameters to obtain the most realistic picture for visual and qualitative evaluation of the biopolymeric scaffold. Moreover, scaffold images were stereoscopically visualized in order to better see the 3D biopolymer composite scaffold morphology. However, the optimized visualization has some discontinuities in zoomed view, which can be problematic for further analysis of interconnected pores by commonly used numerical methods. Therefore, we applied the locally adaptive method to solve discontinuities issue. The combination of contrast agent and imaging techniques presented in this paper help us to better understand the structure and morphology of the biopolymeric scaffold that is crucial in the design of new biomaterials useful in tissue engineering.

CEITEC Central European Institute of Technology Brno University of Technology Purkynova 123 61200 Brno Czech Republic

Institute of Biomedical Engineering FEEC Brno University of Technology Technicka 12 61600 Brno Czech Republic

SCITEG a s Brno Czech Republic

Textile Research Division National Research Centre El Buhouth St P O Box 12311 Cairo Egypt

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