Biphasic response in number of stem cells and endothelial progenitor cells after left ventricular assist device implantation: A 6month follow-up
Language English Country Netherlands Media print-electronic
Document type Journal Article, Observational Study
PubMed
27232919
DOI
10.1016/j.ijcard.2016.05.063
PII: S0167-5273(16)30933-0
Knihovny.cz E-resources
- Keywords
- Endothelial progenitor cells, Microparticles, Stem cells, Vascular function, Ventricular assist device,
- MeSH
- Time Factors MeSH
- Endothelium, Vascular cytology physiology MeSH
- Adult MeSH
- Endothelial Progenitor Cells physiology MeSH
- Stem Cells physiology MeSH
- Middle Aged MeSH
- Humans MeSH
- Longitudinal Studies MeSH
- Young Adult MeSH
- Follow-Up Studies MeSH
- Cell Count trends MeSH
- Heart-Assist Devices trends MeSH
- Prospective Studies MeSH
- Aged MeSH
- Heart Ventricles * MeSH
- Heart Failure blood physiopathology therapy MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Young Adult MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Observational Study MeSH
BACKGROUND: Continuous blood flow could have deleterious effects on endothelium and vascular health. This could have serious consequences in patients with heart failure treated with continuous flow left ventricular assist devices (LVAD). Therefore, we studied effect of LVAD on three circulating vascular biomarkers: stem cells (SC), endothelial progenitor cells (EPC) and microparticles (MP). METHODS: In 23 patients (5 women) with end-stage heart failure, SC, EPC and MP were measured before, and 3 and 6months after implantation of LVAD (HeartMate II). SC were defined using determination of surface antigen expression as mononuclear CD34+/CD45low+ cells and EPC as mononuclear CD34+/CD45low+/KDR+ cells. MP concentrations were determined by ELISA method. RESULTS: Three months after LVAD implantation numbers of SC and EPC significantly decreased (p=0.01 and p=0.001, respectively). On the contrary, between 3rd and 6th month after implantation they significantly increased (p=0.006 and p=0.003, respectively).MP did not change significantly during the study despite exerting similar trend as SC and EPC. CONCLUSIONS: Observed biphasic changes of SC and EPC might reflect two processes. First, shortly after LVAD implantation, improved tissue perfusion could lead to decrease in ischemic stimuli and ensuing decrease of SC and EPC. Second, continuous flow between 3rd and 6th month produced by LVAD could lead to increase of SC and EPC through activation of endothelium. This explanation could be supported also by similar trend in the changes of concentrations of MP.
Department of Cardiac Surgery Institute of Clinical and Experimental Medicine Prague Czech Republic
Department of Cardiology Institute of Clinical and Experimental Medicine Prague Czech Republic
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