Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
27287146
DOI
10.1016/j.msec.2016.05.017
PII: S0928-4931(16)30443-X
Knihovny.cz E-resources
- Keywords
- Chitosan, Immobilization, Lipase, Magnetic fluid, Poly(methacrylic acid),
- MeSH
- Candida enzymology MeSH
- Chitosan chemistry MeSH
- Enzymes, Immobilized chemistry MeSH
- Fungal Proteins chemistry MeSH
- Cells, Immobilized cytology metabolism MeSH
- Polymethacrylic Acids chemistry MeSH
- Lipase chemistry MeSH
- Magnetics * MeSH
- Saccharomyces cerevisiae cytology metabolism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Chitosan MeSH
- Enzymes, Immobilized MeSH
- Fungal Proteins MeSH
- Polymethacrylic Acids MeSH
- Lipase MeSH
- polymethacrylic acid MeSH Browser
A simple procedure for the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid) has been developed. This ferrofluid was used to prepare a novel type of magnetically responsive chitosan-based composite material. Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques. Magnetic chitosan was found to be a perspective material for various bioapplications, especially as a magnetic carrier for immobilization of enzymes and cells. Lipase from Candida rugosa was covalently attached after cross-linking and activation of chitosan using glutaraldehyde. Baker's yeast cells (Saccharomyces cerevisiae) were incorporated into the chitosan composite during its preparation; both biocatalysts were active after reaction with appropriate substrates.
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