Expression of nestin, CD133 and ABCG2 in relation to the clinical outcome in pediatric sarcomas
Language English Country United States Media print
Document type Journal Article
PubMed
27314299
DOI
10.3233/cbm-160623
PII: CBM623
Knihovny.cz E-resources
- Keywords
- Ewing sarcoma, Rhabdomyosarcoma, cancer stem cell markers, osteosarcoma, prognosis,
- MeSH
- ATP Binding Cassette Transporter, Subfamily G, Member 2 genetics metabolism MeSH
- AC133 Antigen genetics metabolism MeSH
- Child MeSH
- Adult MeSH
- Gene Expression MeSH
- Immunohistochemistry MeSH
- Kaplan-Meier Estimate MeSH
- Infant MeSH
- Humans MeSH
- Neoplasm Metastasis MeSH
- Adolescent MeSH
- Young Adult MeSH
- Biomarkers, Tumor * MeSH
- Neoplastic Stem Cells metabolism MeSH
- Nestin genetics metabolism MeSH
- Infant, Newborn MeSH
- Child, Preschool MeSH
- Prognosis MeSH
- Sarcoma diagnosis genetics metabolism mortality MeSH
- Neoplasm Staging MeSH
- Neoplasm Grading MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Infant MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Infant, Newborn MeSH
- Child, Preschool MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- ATP Binding Cassette Transporter, Subfamily G, Member 2 MeSH
- AC133 Antigen MeSH
- Biomarkers, Tumor * MeSH
- Nestin MeSH
BACKGROUND: Nestin, CD133 and ABCG2 are recently discussed as putative markers, co-expression of which might determine a cancer stem cell (CSC) phenotype in sarcomas. OBJECTIVE: Our study is focused on immunohistochemical analysis of nestin, CD133 and ABCG2 expression in rhabdomyosarcoma, Ewing sarcoma and osteosarcoma. Furthermore, we also analyzed the possible correlation of nestin, CD133 and ABCG2 expression levels with the patient outcome to identify potential prognostic values of these three putative CSC markers in the same cohorts. METHODS: Using immunohistochemistry, expression of nestin, CD133 and ABCG2 was analyzed in 24 rhabdomyosarcoma, 22 Ewing sarcoma and 10 osteosarcoma tissue samples and expression levels of these markers were correlated with clinical outcome. RESULTS: High nestin levels indicate poor prognosis in patients with Ewing sarcoma (P = 0.001), and high CD133 expression is associated with shorter survival in rhabdomyosarcoma patients (P = 0.002). In contrast, no significant relationship was found between ABCG2 expression and the clinical outcome. CONCLUSIONS: Our analysis represents the first complex study of these three putative CSCs markers together in three different types of pediatric sarcomas and showed their possible prognostic values in these tumors.
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