Molecular survey of arthropod-borne pathogens in sheep keds (Melophagus ovinus), Central Europe
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27325400
DOI
10.1007/s00436-016-5175-2
PII: 10.1007/s00436-016-5175-2
Knihovny.cz E-zdroje
- Klíčová slova
- Arboviruses, Babesia spp., Bartonella melophagi, Borrelia burgdorferi, Melophagus ovinus, Rickettsiae,
- MeSH
- Anaplasma phagocytophilum genetika izolace a purifikace MeSH
- Anaplasmataceae genetika izolace a purifikace MeSH
- arboviry genetika izolace a purifikace MeSH
- Babesia genetika izolace a purifikace MeSH
- Bartonella genetika izolace a purifikace MeSH
- Diptera mikrobiologie MeSH
- nemoci ovcí epidemiologie mikrobiologie MeSH
- ovce MeSH
- průzkumy a dotazníky MeSH
- Rickettsia genetika izolace a purifikace MeSH
- sekvenční analýza DNA veterinární MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
In the study, we screened a total of 399 adult sheep keds (Melophagus ovinus) for the presence of RNA and DNA specific for arboviral, bacterial, and protozoan vector-borne pathogens. All investigated keds were negative for flaviviruses, phleboviruses, bunyaviruses, Borrelia burgdorferi, Rickettsia spp., Anaplasma phagocytophilum, "Candidatus Neoehrlichia mikurensis," and Babesia spp. All ked pools were positive for Bartonella DNA. The sequencing of the amplified fragments of the gltA and 16S-23S rRNA demonstrated a 100 % homology with Bartonella melophagi previously isolated from a sheep ked and from human blood in the USA. The identification of B. melophagi in sheep keds in Central Europe highlights needs extending a list of hematophagous arthropods beyond ticks and mosquitoes for a search of emerging arthropod-borne pathogens.
Department of Experimental Biology Masaryk University Kotlarska 2 611 37 Brno Czech Republic
Institute of Vertebrate Biology v v i Academy of Sciences Kvetna 8 CZ 603 65 Brno Czech Republic
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