Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
27345169
DOI
10.1016/j.dci.2016.06.015
PII: S0145-305X(16)30196-3
Knihovny.cz E-resources
- Keywords
- CD11c, Dendritic cells, Mononuclear phagocytes, Pig model, XCR1,
- MeSH
- CD11c Antigen immunology metabolism MeSH
- Dendritic Cells physiology MeSH
- Phagocytes immunology MeSH
- Immunologic Tests methods MeSH
- Cloning, Molecular MeSH
- Skin metabolism MeSH
- Humans MeSH
- Disease Models, Animal MeSH
- Antibodies, Monoclonal metabolism MeSH
- Mononuclear Phagocyte System * MeSH
- Swine immunology MeSH
- Receptors, G-Protein-Coupled immunology metabolism MeSH
- Veterinary Medicine MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CD11c Antigen MeSH
- Antibodies, Monoclonal MeSH
- Receptors, G-Protein-Coupled MeSH
Pig is a domestic species of major importance in the agro-economy and in biomedical research. Mononuclear phagocytes (MNP) are organized in subsets with specialized roles in the orchestration of the immune response and new tools are awaited to improve MNP subset identification in the pig. We cloned pig CD11c cDNA and generated a monoclonal antibody to pig CD11c which showed a pattern of expression by blood and skin MNP subsets similar to humans. We also developed a porcine XCL1-mCherry dimer which specifically reacted with the XCR1-expressing dendritic cell subset of the type 1 lineage in blood and skin. These original reagents will allow the efficient identification of pig MNP subsets to study their role in physiological and pathological processes and also to target these cells in novel intervention and vaccine strategies for veterinary applications and preclinical evaluations.
Institute of Microbiology of the Czech Academy of Sciences v v i 142 20 Prague Czech Republic
MI mAbs Parc Scientifique et Technologique de Luminy Case 906 F13288 Marseille Cedex 9 France
VIM INRA Université Paris Saclay Domaine de Vilvert 78350 Jouy en Josas France
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