Pig is a domestic species of major importance in the agro-economy and in biomedical research. Mononuclear phagocytes (MNP) are organized in subsets with specialized roles in the orchestration of the immune response and new tools are awaited to improve MNP subset identification in the pig. We cloned pig CD11c cDNA and generated a monoclonal antibody to pig CD11c which showed a pattern of expression by blood and skin MNP subsets similar to humans. We also developed a porcine XCL1-mCherry dimer which specifically reacted with the XCR1-expressing dendritic cell subset of the type 1 lineage in blood and skin. These original reagents will allow the efficient identification of pig MNP subsets to study their role in physiological and pathological processes and also to target these cells in novel intervention and vaccine strategies for veterinary applications and preclinical evaluations.

Centre d'Immunologie de Marseille Luminy Aix Marseille Université UM2 Inserm U1104 CNRS UMR7280 13288 Marseille France

Institute of Microbiology of the Czech Academy of Sciences v v i 142 20 Prague Czech Republic

K G Jebsen Center for Research on Influenza Vaccines University of Oslo and Oslo University Hospital 0027 Oslo Norway

K G Jebsen Center for Research on Influenza Vaccines University of Oslo and Oslo University Hospital 0027 Oslo Norway; Center for Immune Regulation Institute of Immunology University of Oslo and Oslo University Hospital Rikshospitalet 0424 Oslo Norway

MI mAbs Parc Scientifique et Technologique de Luminy Case 906 F13288 Marseille Cedex 9 France

VIM INRA Université Paris Saclay Domaine de Vilvert 78350 Jouy en Josas France

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