BACKGROUND: The importance of soil biota in the composition of mature plant communities is commonly acknowledged. In contrast, the role of soil biota in the early establishment of new plant communities and their relative importance for soil abiotic conditions are still poorly understood. AIMS AND METHODS: The aim of this study was to understand the effects of soil origin and soil fungal communities on the composition of a newly established dry grassland plant community. We used soil from two different origins (dry grassland and abandoned field) with different pH and nutrient and mineral content. Grassland microcosms were established by sowing seeds of 54 species of dry grassland plants into the studied soils. To suppress soil fungi, half of the pots were regularly treated with fungicide. In this way, we studied the independent and combined effects of soil origin and soil community on the establishment of dry grassland communities. KEY RESULTS: The effect of suppressing the soil fungal community on the richness and composition of the plant communities was much stronger than the effect of soil origin. Contrary to our expectations, the effects of these two factors were largely additive, indicating the same degree of importance of soil fungal communities in the establishment of species-rich plant communities in the soils from both origins. The negative effect of suppressing soil fungi on species richness, however, occurred later in the soil from the abandoned field than in the soil from the grassland. This result likely occurred because the negative effects of the suppression of fungi in the field soil were caused mainly by changes in plant community composition and increased competition. In contrast, in the grassland soil, the absence of soil fungi was limiting for plants already at the early stages of their establishment, i.e., in the phases of germination and early recruitment. While fungicide affects not only arbuscular mycorrhizal fungi but also other biota, our data indicate that changes in the AMF communities are the most likely drivers of the observed changes. The effects of other soil biota, however, cannot be fully excluded. CONCLUSIONS: These results suggest that the availability of soil fungi may not be the most important limiting factor for the establishment of grassland species in abandoned fields if we manage to reduce the intensity of competition at these sites e.g., by mowing or grazing.

Department of Botany Faculty of Science Charles University Prague Benátská 2 128 01 Prague Czech Republic

Institute of Botany Academy of Sciences Zámek 1 252 43 Průhonice Czech Republic

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Reeves FB, Wagner D, Moorman T, Kiel J. Role of endomycorrhizae in revegetation practices in the semi-arid west .1. Comparison of incidence of mycorrhizae in severely disturbed vs natural environments. American Journal of Botany. 1979;66(1):6–13. 10.2307/2442618 . DOI

Janos DP. Mycorrhizae influence tropical succession. Biotropica. 1980;12:56–64. 10.2307/2388157 . DOI

Puschel D, Rydlova J, Vosatka M. Mycorrhiza influences plant community structure in succession on spoil banks. Basic and Applied Ecology. 2007;8(6):510–20. 10.1016/j.baae.2006.09.002 . DOI

van Breugel M, Hall JS, Craven D, Bailon M, Hernandez A, Abbene M, et al. Succession of Ephemeral Secondary Forests and Their Limited Role for the Conservation of Floristic Diversity in a Human-Modified Tropical Landscape. Plos One. 2013;8(12):13 10.1371/journal.pone.0082433 . PubMed DOI PMC

Tognetti PM, Chaneton EJ. Invasive exotic grasses and seed arrival limit native species establishment in an old-field grassland succession. Biological Invasions. 2012;14(12):2531–44. 10.1007/s10530-012-0249-2 . DOI

Standish RJ, Cramer VA, Wild SL, Hobbs RJ. Seed dispersal and recruitment limitation are barriers to native recolonization of old-fields in western Australia. Journal of Applied Ecology. 2007;44(2):435–45. 10.1111/j.1365-2664.2006.01262.x . DOI

Lindborg R. Recreating grasslands in Swedish rural landscapes—effects of seed sowing and management history. Biodiversity and Conservation. 2006;15(3):957–69. 10.1007/s10531-004-3508-4 . DOI

Foster BL, Kindscher K, Houseman GR, Murphy CA. Effects of hay management and native species sowing on grassland community structure, biomass, and restoration. Ecological Applications. 2009;19(7):1884–96. 10.1890/08-0849.1 . PubMed DOI

Münzbergová Z, Herben T. Identification of suitable unoccupied habitats in metapopulation studies using co-occurrence of species. Oikos. 2004;105(2):408–14. 10.1111/j.0030-1299.2004.13017.x . DOI

Osbornová J, Kovářová M, Lepš J, Prach K (Eds.),. Succession in Abandoned Fields: Studies in Central Bohemia, Czechoslovakia. Dordrecht; London: Kluwer Academic,; 1990.

Pywell RF, Bullock JM, Hopkins A, Walker KJ, Sparks TH, Burke MJW, et al. Restoration of species-rich grassland on arable land: assessing the limiting processes using a multi-site experiment. J Appl Ecol. 2002;39(2):294–309. .

Walker KJ, Stevens PA, Stevens DP, Mountford JO, Manchester SJ, Pywell RF. The restoration and re-creation of species-rich lowland grassland on land formerly managed for intensive agriculture in the UK. Biol Conserv. 2004;119(1):1–18. .

Chylova T, Münzbergová Z. Past land use co-determines the present distribution of dry grassland plant species. Preslia. 2008;80(2):183–98. .

Knappova J, Knapp M, Münzbergová Z. Spatio-Temporal Variation in Contrasting Effects of Resident Vegetation on Establishment, Growth and Reproduction of Dry Grassland Plants: Implications for Seed Addition Experiments. Plos One. 2013;8(6). 10.1371/journal.pone.0065879 . PubMed DOI PMC

Hemrova L, Münzbergová Z. The effects of plant traits on species' responses to present and historical patch configurations and patch age. Oikos. 2015;124(4):437–45. 10.1111/oik.01130 . DOI

Knappová J, Hemrová L, Münzbergová Z. Colonization of central European abandoned fields by dry grassland species depends on the species richness of the source habitats: a new approach for measuring habitat isolation. Landsc Ecol. 2012;27(1):97–108.

Knappova J, Münzbergová Z. Low seed pressure and competition from resident vegetation restricts dry grassland specialists to edges of abandoned fields. Agriculture Ecosystems & Environment. 2015;200:200–7. 10.1016/j.agee.2014.11.008 . DOI

Janssens F, Peeters A, Tallowin JRB, Bakker JP, Bekker RM, Fillat F, et al. Relationship between soil chemical factors and grassland diversity. Plant and Soil. 1998;202(1):69–78. 10.1023/a:1004389614865 . DOI

Raabova J, Münzbergová Z, Fischer M. The role of spatial scale and soil for local adaptation in Inula hirta. Basic and Applied Ecology. 2011;12(2):152–60. 10.1016/j.baae.2011.01.001 . DOI

Yang H, Li Y, Wu M, Zhang Z, Li L, Wan S. Plant community responses to nitrogen addition and increased precipitation: the importance of water availability and species traits. Global Change Biology. 2011;17(9):2936–44. 10.1111/j.1365-2486.2011.02423.x . DOI

Kardol P, Souza L, Classen AT. Resource availability mediates the importance of priority effects in plant community assembly and ecosystem function. Oikos. 2013;122(1):84–94. 10.1111/j.1600-0706.2012.20546.x . DOI

Cerna L, Münzbergová Z. Conditions in Home and Transplant Soils Have Differential Effects on the Performance of Diploid and Allotetraploid Anthericum Species. Plos One. 2015;10(1). 10.1371/journal.pone.0116992 . PubMed DOI PMC

Kardol P, Dickie IA, St John MG, Husheer SW, Bonner KI, Bellingham PJ, et al. Soil-mediated effects of invasive ungulates on native tree seedlings. Journal of Ecology. 2014;102(3):622–31. 10.1111/1365-2745.12234 . DOI

Massenssini AM, Bonduki VHA, Melo CAD, Totola MR, Ferreira FA, Costa MD. Soil microorganisms and their role in the interactions between weeds and crops. Planta Daninha. 2014;32(4):873–84. .

McGonigle TP, Evans DG, Miller MH. Effect of degree of soil disturbance on mycorrhizal colonization and phosphorus absorption by maize in growth chamber and field experiments. New Phytologist. 1990;116(4):629–36. 10.1111/j.1469-8137.1990.tb00548.x . DOI

van der Wal A, de Boer W, Gunnewiek P, van Veen JA. Possible Mechanism for Spontaneous Establishment of Calluna vulgaris in a Recently Abandoned Agricultural Field. Restoration Ecology. 2009;17(2):308–13. 10.1111/j.1526-100X.2007.00349.x . DOI

Stover HJ, Thorn RG, Bowles JM, Bernards MA, Jacobs CR. Arbuscular mycorrhizal fungi and vascular plant species abundance and community structure in tallgrass prairies with varying agricultural disturbance histories. Applied Soil Ecology. 2012;60:61–70. 10.1016/j.apsoil.2012.02.016 . DOI

Duchicela J, Sullivan TS, Bontti E, Bever JD. Soil aggregate stability increase is strongly related to fungal community succession along an abandoned agricultural field chronosequence in the Bolivian Altiplano. Journal of Applied Ecology. 2013;50(5):1266–73. 10.1111/1365-2664.12130 . DOI

Gryndler M, Lipavsky J. Effect of phosphate fertilization on the populations of arbuscular mycorrhizal fungi. Rostlinna Vyroba. 1995;41(11):533–40. .

Verbruggen E, Kiers ET. Evolutionary ecology of mycorrhizal functional diversity in agricultural systems. Evolutionary Applications. 2010;3(5–6):547–60. 10.1111/j.1752-4571.2010.00145.x . PubMed DOI PMC

Doubkova P, Vlasakova E, Sudova R. Arbuscular mycorrhizal symbiosis alleviates drought stress imposed on Knautia arvensis plants in serpentine soil. Plant and Soil. 2013;370(1–2):149–61. 10.1007/s11104-013-1610-7 . DOI

Smith SE, Read DJ. Mycorrhizal Symbiosis, 3rd Edition. Mycorrhizal Symbiosis, 3rd Edition. 2008:1–787. .

Boyer LR, Brain P, Xu X-M, Jeffries P. Inoculation of drought-stressed strawberry with a mixed inoculum of two arbuscular mycorrhizal fungi: effects on population dynamics of fungal species in roots and consequential plant tolerance to water deficiency. Mycorrhiza. 2015;25(3):215–27. 10.1007/s00572-014-0603-6 . PubMed DOI

Zhao R, Guo W, Bi N, Guo J, Wang L, Zhao J, et al. Arbuscular mycorrhizal fungi affect the growth, nutrient uptake and water status of maize (Zea mays L.) grown in two types of coal mine spoils under drought stress. Applied Soil Ecology. 2015;88:41–9. 10.1016/j.apsoil.2014.11.016 . DOI

van der Heijden MGA, Boller T, Wiemken A, Sanders IR. Different arbuscular mycorrhizal fungal species are potential determinants of plant community structure. Ecology. 1998;79(6):2082–91. 10.1890/0012-9658(1998)079[2082:damfsa]2.0.co;2 . DOI

Smith MD, Hartnett DC, Wilson GWT. Interacting influence of mycorrhizal symbiosis and competition on plant diversity in tallgrass prairie. Oecologia. 1999;121(4):574–82. 10.1007/s004420050964 . PubMed DOI

Callaway RM. Positive interactions among plants. Botanical Review. 1995;61(4):306–49. 10.1007/bf02912621 . DOI

van der Heijden MGA, Wiemken A, Sanders IR. Different arbuscular mycorrhizal fungi alter coexistence and resource distribution between co-occurring plant. New Phytologist. 2003;157(3):569–78. 10.1046/j.1469-8137.2003.00688.x . PubMed DOI

Hart MM, Reader RJ, Klironomos JN. Plant coexistence mediated by arbuscular mycorrhizal fungi. Trends in Ecology & Evolution. 2003;18(8):418–23. 10.1016/s0169-5347(03)00127-7 . DOI

Dostalek T, Pankova H, Münzbergová Z, Rydlova J. The Effect of AMF Suppression on Plant Species Composition in a Nutrient-Poor Dry Grassland. Plos One. 2013;8(11). 10.1371/journal.pone.0080535 . PubMed DOI PMC

Read DJ, Birch CPD. The effects and implications of disturbance of mycorrhizal mycelial systems. Proceedings of the Royal Society of Edinburgh Section B-Biological Sciences. 1988;94:13–24. .

Pankova H, Münzbergová Z, Rydlova J, Vosatka M. The response of aster amellus (asteraceae) to mycorrhiza depends on the origins of both the soil and the fungi. American Journal of Botany. 2011;98(5):850–8. 10.3732/ajb.0900350 . PubMed DOI

Oehl F, Sieverding E, Ineichen K, Mader P, Boller T, Wiemken A. Impact of land use intensity on the species diversity of arbuscular mycorrhizal fungi in agroecosystems of Central Europe. Applied and Environmental Microbiology. 2003;69(5):2816–24. 10.1128/aem.69.5.2816-2824.2003 . PubMed DOI PMC

Mader P, Edenhofer S, Boller T, Wiemken A, Niggli U. Arbuscular mycorrhizae in a long-term field trial comparing low-input (organic, biological) and high-input (conventional) farming systems in a crop rotation. Biology and Fertility of Soils. 2000;31(2):150–6. 10.1007/s003740050638 . DOI

Oehl F, Sieverding E, Ineichen K, Ris EA, Boller T, Wiemken A. Community structure of arbuscular mycorrhizal fungi at different soil depths in extensively and intensively managed agroecosystems. New Phytologist. 2005;165(1):273–83. 10.1111/j.1469-8137.2004.01235.x . PubMed DOI

Hijri I, Sykorova Z, Oehl F, Ineichen K, Mader P, Wiemken A, et al. Communities of arbuscular mycorrhizal fungi in arable soils are not necessarily low in diversity. Molecular Ecology. 2006;15(8):2277–89. 10.1111/j.1365-294X.2006.02921.x . PubMed DOI

Johnson NC, Zak DR, Tilman D, Pfleger FL. Dynamics of vesicular-arbuscular mycorrhizae during old field succession. Oecologia. 1991;86(3):349–58. 10.1007/bf00317600 . PubMed DOI

Johnson NC. Can fertilization of soil select less mutualistic mycorrhizae. Ecological Applications. 1993;3(4):749–57. 10.2307/1942106 . PubMed DOI

Heneghan L, Miller SP, Baer S, Callaham MA, Montgomery J, Pavao-Zuckerman M, et al. Integrating Soil Ecological Knowledge into Restoration Management. Restoration Ecology. 2008;16(4):608–17. 10.1111/j.1526-100X.2008.00477.x . DOI

Eriksson O, Eriksson A. Effects of arrival order and seed size on germination of grassland plants: Are there assembly rules during recruitment? Ecological Research. 1998;13(2):229–39. 10.1046/j.1440-1703.1998.00260.x . DOI

Fukami T, Bezemer TM, Mortimer SR, van der Putten WH. Species divergence and trait convergence in experimental plant community assembly. Ecology Letters. 2005;8(12):1283–90. 10.1111/j.1461-0248.2005.00829.x . DOI

Fukami T, Nakajima M. Community assembly: alternative stable states or alternative transient states? Ecology Letters. 2011;14(10):973–84. 10.1111/j.1461-0248.2011.01663.x . PubMed DOI PMC

Münzbergová Z. Seed Density Significantly Affects Species Richness and Composition in Experimental Plant Communities. Plos One. 2012;7(10). 10.1371/journal.pone.0046704 . PubMed DOI PMC

Newsham K, Fitter A, Watkinson A. Root pathogenic and arbuscular mycorrhizal fungi determine fecundity of asymptomatic plants in the field. Journal of Ecology. 1994;82(4):805–14. 10.2307/2261445 . DOI

Knappova J, Hemrova L, Münzbergová Z. Colonization of central European abandoned fields by dry grassland species depends on the species richness of the source habitats: a new approach for measuring habitat isolation. Landscape Ecology. 2012;27(1):97–108. 10.1007/s10980-011-9680-5 . DOI

Sakai S, Harada Y. Size-number trade-off and optimal offspring size for offspring produced sequentially using a fixed amount of reserves. Journal of Theoretical Biology. 2004;226(3):253–64. 10.1016/s0022-5193(03)00281-9 . PubMed DOI

Mouquet N, Leadley P, Meriguet J, Loreau M. Immigration and local competition in herbaceous plant communities: a three-year seed-sowing experiment. Oikos. 2004;104(1):77–90. 10.1111/j.0030-1299.2004.12644.x . DOI

Knappová J, Knapp M, Münzbergová Z. Spatio-Temporal Variation in Contrasting Effects of Resident Vegetation on Establishment, Growth and Reproduction of Dry Grassland Plants: Implications for Seed Addition Experiments. Plos One. 2013;8(6). 10.1371/journal.pone.0065879 . PubMed DOI PMC

Jakobsen I, Nielsen NE. Vesicular arbuscular mycorrhiza in field-grown crops .1. Mycorrhizal infection in cereals and peas at various times and soil depths. New Phytologist. 1983;93(3):401–13. .

An ZQ, Grove JH, Hendrix JW, Hershman DE, Henson GT. Vertical-distribution of endogonaceous mycorrhizal fungi associated with soybean, as affected by soil fumigation. Soil Biology & Biochemistry. 1990;22(5):715–9. 10.1016/0038-0717(90)90020-z . DOI

Kabir Z, O'Halloran IP, Widden P, Hamel C. Vertical distribution of arbuscular mycorrhizal fungi under corn (Zea mays L.) in no-till and conventional tillage systems. Mycorrhiza. 1998;8(1):53–5. 10.1007/s005720050211 . DOI

Hoechst Schering AgrEvo. Technical Information—Carbendazim GmbH, Product marketing Fungicides. Frankfurt/Main, Germany: Bayer CropScience.

Environmental Protection Agency. Benomyl; Cancellation Order. USA2001.

Schweiger P, Spliid N, Jakobsen I. Fungicide application and phosphorus uptake by hyphae of arbuscular mycorrhizal fungi into field-grown peas. Soil Biology & Biochemistry. 2001;33(9):1231–7. 10.1016/S0038-0717(01)00028-1 . DOI

Hartnett D, Wilson G. Mycorrhizae influence plant community structure and diversity in tallgrass prairie. Ecology. 1999;80(4):1187–95. 10.1890/0012-9658(1999)080[1187:MIPCSA]2.0.CO;2 . DOI

O'Connor P, Smith S, Smith F. Arbuscular mycorrhizas influence plant diversity and community structure in a semiarid herbland. New Phytologist. 2002;154(1):209–18. 10.1046/j.1469-8137.2002.00364.x . DOI

Fitter A. Effect of benomyl on leaf phosphorus concentration in alpine grasslands—a test of mycorrhizal benefit. New Phytologist. 1986;103(4):767–76. 10.1111/j.1469-8137.1986.tb00851.x . DOI

Helgason T, Merryweather J, Young J, Fitter A. Specificity and resilience in the arbuscular mycorrhizal fungi of a natural woodland community. Journal of Ecology. 2007;95(4):623–30. 10.1111/j.1365-2745.2007.01239.x . DOI

Dhillion S, Gardsjord T. Arbuscular mycorrhizas influence plant diversity, productivity, and nutrients in boreal grasslands. Canadian Journal of Botany-Revue Canadienne De Botanique. 2004;82(1):104–14. 10.1139/B03-139 . DOI

Olsen R, Cole C, Watanabe F, Dean L. Estimation of available phosphorus in soils by extraction with podium bicarbonate. US Department of Agriculture Circular. 1954;939:1–19.

Koske R, Gemma J. A modified procedure for staining roots to detect VA-mycorrhizas. Mycological Research. 1989;92:486–505. .

Giovannetti M, Mosse B. Evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytologist. 1980;84(3):489–500. 10.1111/j.1469-8137.1980.tb04556.x . DOI

Wilson J, Trinick M. Factors affecting the estimation of numbers of infective propagules of vesicular arbuscular mycorrhizal fungi by the most probable number method. Australian Journal of Soil Research. 1983;21(1):73–81. 10.1071/SR9830073 . DOI

Pankova H, Münzbergová Z, Rydlova J, Vosatka M. Differences in AM fungal root colonization between populations of perennial Aster species have genetic reasons. Oecologia. 2008;157(2):211–20. 10.1007/s00442-008-1064-4 . PubMed DOI

ter Braak C, Šmilauer P. Canoco reference manual and users guide to Canoco forWindows: Software for canonical community ordination (version 4) Ithaca: Microcomputer Power; 1998.

Lepš J, Šmilauer P. Multivariate Analysis Ecological Data using CANOCO: Cambridge University Press; 2003. 269 p.

Martensson AM. Effects of agrochemicals and heavy-metals on fast-growing rhizobia and their symbiosis with small-seeded legumes. Soil Biology & Biochemistry. 1992;24(5):435–45. 10.1016/0038-0717(92)90206-d . DOI

Drouin P, Sellami M, Prevost D, Fortin J, Antoun H. Tolerance to agricultural pesticides of strains belonging to four genera of Rhizobiaceae. Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Wastes. 2010;45(8):757–65. 10.1080/03601234.2010.515168 . PubMed DOI

Voříšková A, Janoušková M, Slavíková R, Pánková H, Daniel O, Vazačová K, et al. Effect of past agricultural use on the infectivity and composition of a community of arbuscular mycorrhizal fungi. Agriculture, Ecosystems & Environment. 2016;221:28–39. 10.1016/j.agee.2016.01.012. DOI

Gange A, Brown V, Sinclair G. Vesicular-arbuscular mycorrhizal fungi: a determinant of plant community structure in early succession. Functional Ecology. 1993;7(5):616–22. 10.2307/2390139 . DOI

Karanika E, Mamolos A, Alifragis D, Kalburtji K, Veresoglou D. Arbuscular mycorrhizas contribution to nutrition, productivity, structure and diversity of plant community in mountainous herbaceous grassland of northern Greece. Plant Ecology. 2008;199(2):225–34. 10.1007/s11258-008-9427-9 . DOI

Hart MM, Reader RJ. Taxonomic basis for variation in the colonization strategy of arbuscular mycorrhizal fungi. New Phytologist. 2002;153(2):335–44. 10.1046/j.0028-646X.2001.00312.x . DOI

Middleton EL, Bever JD. Inoculation with a Native Soil Community Advances Succession in a Grassland Restoration. Restoration Ecology. 2012;20(2):218–26. 10.1111/j.1526-100X.2010.00752.x . DOI

Ejrnaes R, Bruun HH, Graae BJ. Community assembly in experimental Grasslands: Suitable environment or timely arrival? Ecology. 2006;87(5):1225–33. 10.1890/0012-9658(2006)87[1225:caiegs]2.0.co;2 . PubMed DOI

van der Heijden MGA. Arbuscular mycorrhizal fungi as support systems for seedling establishment in grassland. Ecology Letters. 2004;7(4):293–303. 10.1111/j.1461-0248.2004.00577.x . DOI

Smith M, Hartnett D, Wilson G. Interacting influence of mycorrhizal symbiosis and competition on plant diversity in tallgrass prairie. Oecologia. 1999;121(4):574–82. 10.1007/s004420050964 . PubMed DOI

Bever JD. Soil community feedback and the coexistence of competitors: conceptual frameworks and empirical tests. New Phytologist. 2003;157(3):465–73. 10.1046/j.1469-8137.2003.00714.x . PubMed DOI

Harley J, Harley E. A checklist of mycorrhiza in the british flora—addenda, errata and index. New Phytologist. 1987;107(4):741–9. 10.1111/j.1469-8137.1987.tb00912.x . DOI

Muthukumar T, Udaiyan K, Shanmughavel P. Mycorrhiza in sedges—an overview. Mycorrhiza. 2004;14(2):65–77. 10.1007/s00572-004-0296-3 . PubMed DOI

Grime J, Mackey J, Hillier S, Read D. Floristic diversity in a model system using experimental microcosms. Nature. 1987;328(6129):420–2. 10.1038/328420a0 . DOI

Wilson G, Hartnett D. Interspecific variation in plant responses to mycorrhizal colonization in tallgrass prairie. American Journal of Botany. 1998;85(12):1732–8. 10.2307/2446507 . PubMed DOI

Mouillot D, Mouquet N. Species richness peaks for intermediate levels of biomass in a fractal succession with quasi-neutral interactions. Oikos. 2006;115(2):349–57. 10.1111/j.2006.0030-1299.14894.x . DOI

Poggio SL, Ghersa CM. Species richness and evenness as a function of biomass in arable plant communities. Weed Research. 2011;51(3):241–9. 10.1111/j.1365-3180.2011.00845.x . DOI

Aerts R, Boot RGA, Vanderaart PJM. The relation between aboveground and belowground biomass allocation patterns and competitive ability. Oecologia. 1991;87(4):551–9. 10.1007/bf00320419 . PubMed DOI

Skalova H, Krahulec F, During HJ, Hadincova V, Pechackova S, Herben T. Grassland canopy composition and spatial heterogeneity in the light quality. Plant Ecology. 1999;143(2):129–39. 10.1023/a:1009899803229 . DOI

Gange AC, Ayres RL. On the relation between arbuscular mycorrhizal colonization and plant 'benefit'. Oikos. 1999;87(3):615–21. 10.2307/3546829 . DOI

Anderson RC. Growth and Arbuscular Mycorrhizal Fungal Colonization of Two Prairie Grasses Grown in Soil from Restorations of Three Ages. Restoration Ecology. 2008;16(4):650–6. 10.1111/j.1526-100X.2008.00488.x . DOI

Johnson NC, Pfleger FL. Vesicular-arbuscular mycorrhizae and cultural stresses. Mycorrhizae in Sustainable Agriculture. 1992;54:71–99. .

Johnson NC, Pfleger FL, Crookston RK, Simmons SR, Copeland PJ. Vesicular arbuscular mycorrhizas respond to corn and soybean cropping history. New Phytologist. 1991;117(4):657–63. 10.1111/j.1469-8137.1991.tb00970.x . DOI

Boddington CL, Dodd JC. The effect of agricultural practices on the development of indigenous arbuscular mycorrhizal fungi. I. Field studies in an Indonesian ultisol. Plant and Soil. 2000;218(1–2):137–44. 10.1023/a:1014966801446 . DOI

Boddington CL, Dodd JC. The effect of agricultural practices on the development of indigenous arbuscular mycorrhizal fungi. II. Studies in experimental microcosms. Plant and Soil. 2000;218(1–2):145–57. 10.1023/a:1014911318284 . DOI

Merryweather J, Fitter A. Phosphorus nutrition of an obligately mycorrhizal plant treated with the fungicide benomyl in the field. New Phytologist. 1996;132(2):307–11. 10.1111/j.1469-8137.1996.tb01850.x . DOI

Richter BS, Tiller RL, Stutz JC. Assessment of arbuscular mycorrhizal fungal propagules and colonization from abandoned agricultural fields and semi-arid grasslands in riparian floodplains. Applied Soil Ecology. 2002;20(3):227–38. 10.1016/s0929-1393(02)00038-0 . DOI

Boerner REJ, DeMars BG, Leicht PN. Spatial patterns of mycorrhizal infectiveness of soils long a successional chronosequence. Mycorrhiza. 1996;6(2):79–90. 10.1007/s005720050111 . DOI

Barni E, Siniscalco C. Vegetation dynamics and arbuscular mycorrhiza in old-field successions of the western Italian Alps. Mycorrhiza. 2000;10(2):63–72. 10.1007/s005720000059 . DOI

Jansa J, Mozafar A, Anken T, Ruh R, Sanders IR, Frossard E. Diversity and structure of AMF communities as affected by tillage in a temperate soil. Mycorrhiza. 2002;12(5):225–34. 10.1007/s005572-002-0163-z . PubMed DOI

Lopez-Garcia A, Azcon-Aguilar C, Barea JM. The interactions between plant life form and fungal traits of arbuscular mycorrhizal fungi determine the symbiotic community. Oecologia. 2014;176(4):1075–86. 10.1007/s00442-014-3091-7 PubMed DOI

Friese CF, Allen MF. The spread of va mycorrhizal fungal hyphae in the soil—inoculum types and external hyphal architecture. Mycologia. 1991;83(4):409–18. 10.2307/3760351 . DOI

Fitter A, Nichols R. The use of benomyl to control infection by vesicular arbuscular mycorrhizal fungi. New Phytologist. 1988;110(2):201–6. 10.1111/j.1469-8137.1988.tb00253.x . DOI

Chen SK, Edwards CA, Subler S. Effects of the fungicides benomyl, captan and chlorothalonil on soil microbial activity and nitrogen dynamics in laboratory incubations. Soil Biology & Biochemistry. 2001;33(14):1971–80. 10.1016/s0038-0717(01)00131-6 . DOI

The role of plant-soil feedback in long-term species coexistence cannot be predicted from its effects on plant performance

Arbuscular mycorrhizal fungi and associated microbial communities from dry grassland do not improve plant growth on abandoned field soil