The effect of silver nanoparticles and silver ions on mammalian and plant cells in vitro
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27456126
DOI
10.1016/j.fct.2016.07.015
PII: S0278-6915(16)30235-6
Knihovny.cz E-zdroje
- Klíčová slova
- Genotoxicity, In vitro cytotoxicity, Mammalian cell, Silver ions, Silver nanoparticles, Solanum spp.,
- MeSH
- apoptóza účinky léků MeSH
- kometový test MeSH
- kovové nanočástice aplikace a dávkování chemie MeSH
- kultivované buňky MeSH
- lidé MeSH
- myši MeSH
- oxidace-redukce MeSH
- peroxidace lipidů účinky léků MeSH
- poškození DNA účinky léků MeSH
- proliferace buněk účinky léků MeSH
- Ramanova spektroskopie MeSH
- reaktivní formy kyslíku metabolismus MeSH
- Solanum lycopersicum cytologie účinky léků MeSH
- stříbro chemie farmakologie MeSH
- techniky in vitro MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- reaktivní formy kyslíku MeSH
- stříbro MeSH
Silver nanoparticles (AgNPs) are the most frequently applied nanomaterials. In our experiments, we tested AgNPs (size 27 nm) manufactured by the Tollens process. Physico-chemical methods (TEM, DLS, AFM and spectrophotometry) were used for characterization and imaging of AgNPs. The effects of AgNPs and Ag(+) were studied in two experimental models (plant and mammalian cells). Human keratinocytes (SVK14) and mouse fibroblasts (NIH3T3) cell lines were selected to evaluate the cytotoxicity and genotoxicity effect on mammalian cells. Higher sensitivity to AgNPs and Ag(+) was observed in NIH3T3 than in SVK14 cells. AgNPs accumulated in the nucleus of NIH3T3 cells, caused DNA damage and increased the number of apoptotic and necrotic cells. Three genotypes of Solanum spp. (S. lycopersicum cv. Amateur, S. chmielewskii, S. habrochaites) were selected to test the toxicity of AgNPs and Ag(+) on the plant cells. The highest values of peroxidase activity and lipid peroxidation were recorded after the treatment of S. habrochaites genotype with AgNPs. Increased ROS levels were likely the reason for observed damaged membranes in S. habrochaites. We found that the cytotoxic and genotoxic effects of AgNPs depend not only on the characteristics of nanoparticles, but also on the type of cells that are treated with AgNPs.
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