Novel members of the adipokinetic hormone family in beetles of the superfamily Scarabaeoidea
Jazyk angličtina Země Rakousko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
27539647
DOI
10.1007/s00726-016-2314-0
PII: 10.1007/s00726-016-2314-0
Knihovny.cz E-zdroje
- Klíčová slova
- Adipokinetic hormone family, Beetles, Geotrupidae, Insects, Liquid chromatography, Lucanidae, Mass spectrometry, Scarabaeidae,
- MeSH
- brouci chemie genetika MeSH
- chromatografie kapalinová MeSH
- hmotnostní spektrometrie MeSH
- hmyzí hormony chemie genetika izolace a purifikace MeSH
- kyselina pyrrolidonkarboxylová analogy a deriváty chemie izolace a purifikace MeSH
- oligopeptidy chemie genetika izolace a purifikace MeSH
- peptidy chemie genetika izolace a purifikace MeSH
- sekvence aminokyselin genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adipokinetic hormone MeSH Prohlížeč
- hmyzí hormony MeSH
- kyselina pyrrolidonkarboxylová MeSH
- oligopeptidy MeSH
- peptidy MeSH
Eight beetle species of the superfamily Scarabaeoidea were investigated with respect to peptides belonging to the adipokinetic hormone (AKH) family in their neurohemal organs, the corpora cardiaca (CC). The following beetle families are represented: Scarabaeidae, Lucanidae, and Geotrupidae. AKH peptides were identified through a heterospecific trehalose-mobilizing bioassay and by sequence analyses, using liquid chromatography coupled to positive electrospray mass spectrometry (LC-ESI-MS) and analysis of the tandem MS2 spectra obtained by collision-induced dissociation. All the beetle species have octapeptide AKHs; some have two AKHs, while others have only one. Novel AKH members were found in Euoniticellus intermedius and Circellium bacchus (family Scarabaeidae), as well as in Dorcus parallelipipedus (family Lucanidae). Two species of the family Geotrupidae and two species of the Scarabaeidae subfamily Cetoniinae contain one known AKH peptide, Melme-CC, while E. intermedius produces a novel peptide code named Euoin-AKH: pEINFTTGWamide. Two AKH peptides were each identified in CC of C. bacchus and D. parallelipipedus: the novel Cirba-AKH: pEFNFSAGWamide and the known peptide, Scade-CC-I in the former, and the novel Dorpa-AKH: pEVNYSPVW amide and the known peptide, Melme-CC in the latter. Kheper bonelli (subfamily Scarabaeinae) also has two AKHs, the known Scade-CC-I and Scade-CC-II. All the novel peptides were synthesized and the amino acid sequence assignments were unequivocally confirmed by co-elution of the synthetic peptides with their natural equivalent, and identical MS parameters of the two forms. The novel synthetic peptides are all active in inducing hypertrehalosemia in cockroaches.
Biology Centre Czech Academy of Sciences 37005 Ceske Budejovice Czech Republic
Department of Biological Sciences University of Cape Town Private Bag Rondebosch 7700 South Africa
Citace poskytuje Crossref.org
