Meiotic oocytes lack classic centrosomes and, therefore, bipolar spindle assembly depends on clustering of acentriolar microtubule-organizing centers (MTOCs) into two poles. However, the molecular mechanism regulating MTOC assembly into two poles is not fully understood. The kinase haspin (also known as GSG2) is required to regulate Aurora kinase C (AURKC) localization at chromosomes during meiosis I. Here, we show that inhibition of haspin perturbed MTOC clustering into two poles and the stability of the clustered MTOCs. Furthermore, we show that AURKC localizes to MTOCs in mouse oocytes. Inhibition of haspin perturbed the localization of AURKC at MTOCs, and overexpression of AURKC rescued the MTOC-clustering defects in haspin-inhibited oocytes. Taken together, our data uncover a role for haspin as a regulator of bipolar spindle assembly by regulating AURKC function at acentriolar MTOCs in oocytes.

Department of Genetics 145 Bevier Road Rutgers The State University of New Jersey Piscataway NJ 08854 8082 USA

Department of Genetics 145 Bevier Road Rutgers The State University of New Jersey Piscataway NJ 08854 8082 USA Theriogenology Department Faculty of Veterinary Medicine Mansoura University 60 Elgomhoria Street 35516 Mansoura Egypt

Institute of Animal Physiology and Genetics Academy of Sciences of the Czech Republic Rumburská 89 277 21 Liběchov Czech Republic

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