Decrease in Methoprene tolerant and Taiman expression reduces juvenile hormone effects and enhances the levels of juvenile hormone circulating in males of the linden bug Pyrrhocoris apterus
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
27570150
DOI
10.1016/j.jinsphys.2016.08.009
PII: S0022-1910(16)30079-8
Knihovny.cz E-resources
- Keywords
- Accessory glands, Adult males, Corpus allatum, Fat body, Fertility, Haemolymph, Hexameric proteins, Juvenile hormone, Methoprene tolerant, RNA interference, Receptors, Survival, Taiman,
- MeSH
- Corpora Allata physiology surgery MeSH
- Gene Knockdown Techniques MeSH
- Hemolymph chemistry MeSH
- Heteroptera genetics physiology MeSH
- Juvenile Hormones blood genetics metabolism MeSH
- RNA Interference MeSH
- Reproduction MeSH
- Signal Transduction * MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Juvenile Hormones MeSH
Juvenile hormone (JH) produced by the corpus allatum (CA) stimulates vitellogenesis and reduces the synthesis of hexamerin proteins in adult females of Pyrrhocoris apterus. At present it is unknown whether the signaling pathway involving the JH receptor gene Methoprene tolerant (Met) and its binding partner Taiman (Tai), regulates the synthesis of accessory gland proteins (ACPs) and hexamerin proteins or effects male survival. Knockdown of genes by injecting Met dsRNA or Tai dsRNA, reduced the amount of ACPs whilst enhancing the amount of hexamerin mRNA in the fat body and the release of hexamerin proteins into haemolymph, as occurs after the ablation of CA. Lifespan was enhanced by injecting Met but not Tai dsRNA. Diapause associated with the natural absence of JH had a stronger effect on all these parameters than the ablation of CA or the knockdown of genes. This indicates there is an additional regulating agent. Both Met and Tai dsRNA induced a several fold increase in JH (JH III skiped bisepoxide) but a concurrent loss of Met or Tai disabled its function. This supports the view that the Met/Tai complex functions as a JH receptor in the regulation of ACPs and hexamerins.
Biology Center Academy of Sciences of the Czech Republic 37005 Ceske Budejovice Czech Republic
Faculty of Sciences University of South Bohemia 37005 Ceske Budejovice Czech Republic
References provided by Crossref.org
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