Population of wild boar is increasing in the whole Europe, the animals migrate close to human habitats which greatly increases the possibility of natural transmission between domestic animals or humans and wild boars. The aim of the study was to estimate in population of free-living wild boar in the Czech Republic the prevalence of enteric viral pathogens, namely rotavirus groups A and C (RVA and RVC), porcine reproductive and respiratory syndrome virus (PRRSV), and members of family Coronaviridae (transmissible gastroenteritis virus - TGEV, porcine epidemic diarrhea virus - PEDV, porcine respiratory coronavirus - PRCV, and porcine hemagglutination encephalomyelitis virus - PHEV) and Picornaviridae,(teschovirus A - PTV, sapelovirus A - PSV, and enterovirus G - EV-G). In our study, stool samples from 203 wild boars culled during hunting season 2014-2015 (from October to January) were examined by RT-PCR. RVA was detected in 2.5% of tested samples. Nucleotide analysis of VP7, VP4, and VP6 genes revealed that four RVA strains belong to G4P[25]I1, G4P[6]I5, G11P[13]I5, and G5P[13]I5 genotypes and phylogenetic analysis suggested close relation to porcine and human RVAs. The prevalence of RVC in wild boar population reached 12.8%, PTV was detected in 20.2%, PSV in 8.9%, and EV-G in 2.5% of samples. During our study no PRRSV or coronaviruses were detected. Our study provides the first evidence of RVC prevalence in wild boars and indicates that wild boars might contribute to the genetic variability of RVA and also serve as an important reservoir of other enteric viruses.

Department of Forest Protection and Wildlife Management Faculty of Forestry and Wood Technology Mendel University in Brno Zemědělská 1 61300 Brno Czech Republic

Department of Virology Veterinary Research Institute Hudcova 70 62100 Brno Czech Republic

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Amimo J.O., Vlasova A.N., Saif L.J. Prevalence and genetic heterogeneity of porcine group C rotaviruses in nursing and weaned piglets in Ohio, USA and identification of a potential new VP4 genotype. Vet. Microbiol. 2013;164:27–38. PubMed PMC

Cano-Goméz C., García-Casado M., Soriguer R., Palero F., Jiménez-Clavero M.A. Teschoviruses and sapeloviruses in faecal samples from wild boar in Spain. Vet. Microbiol. 2013;165:115–122. PubMed

Cano-Manuel F.J., López-Olvera J., Fandos P., Soriguer R.C., Pérez J.M., Granados J.E. Long-term monitoring of 10 selected pathogens in wild boar (Sus scrofa) in Sierra Nevada National Park, southern Spain. Vet. Microbiol. 2014;174:148–154. PubMed

Fong T.-T., Lipp E.K. Enteric viruses of humans and animals in aquatic environments: health risks, detection, and potential water quality assessment tools. Microbiol. Mol. Biol. Rev. 2005;69:357–371. PubMed PMC

Gabbay Y.B., Borges A.A., Oliveira D.S., Linhares A.C., Mascarenhas J.D., Barardi C.R., Simões C.M., Wang Y., Glass R.I., Jiang B. Evidence for zoonotic transmission of group C rotaviruses among children in Belém, Brazil. J. Med. Virol. 2008;80:1666–1674. PubMed

Ghosh S., Kobayashi N. Whole-genomic analysis of rotavirus strains: current status and future prospects. Future Microbiol. 2011;6:1049–1065. PubMed

Gonzáles-Barrio D., Martín-Hernando M.P., Ruiz-Fons F. Shedding patterns of endemic Eurasian wild boar (Sus scrofa) pathogens. Res. Vet. Sci. 2015;102:206–211. PubMed

Hälli O., Ala-Kurikka E., Nokireki T., Skrypczak T., Raunio-Saarnisto M., Peltoniemi O.A., Heinonen M. Prevalence and risk factors associated with viral and bacterial pathogens in farmed European wild boar. Vet. J. 2012;194:98–101. PubMed PMC

Jeong Y.J., Park S.I., Hosmillo M., Shin D.J., Chun Y.H., Kim H.J., Kwon H.J., Kang S.Y., Woo S.K., Park S.J., Kim G.Y., Kang M.I., Cho K.O. Detection and molecular characterization of porcine group C rotaviruses in South Korea. Vet. Microbiol. 2009;138:217–224. PubMed PMC

Kimura M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 1980;16:111–120. PubMed

Knowles N.J. Porcine enteric picornaviruses. In: Straw B.E., Zimmermann J.J., D́Allaire S.D., Taylor D.J., editors. Diseases of Swine. ninth ed. Blackwell Publishing Inc.; Ames: 2006. pp. 337–345.

Maes P., Matthijnssens J., Rahman M., Van Ranst M. RotaC: a web-based tool for the complete genome classification of group A rotaviruses. BMC Microbiol. 2009;9:238. PubMed PMC

Malenovska H. Virus quantitation by transmission electron microscopy TCID50, and the role of timing virus harvesting: a case study of three animal viruses. J. Virol. Methods. 2013;191:136–140. PubMed

Martella V., Bányai K., Lorusso E., Decaro N., Bellacicco A., Desario C., Corrente M., Greco G., Moschidou P., Tempesta M., Arista S., Ciarlet M., Lavazza A., Buonavoglia C. Prevalence of group C rotaviruses in weaning and post-weaning pigs with enteritis. Vet. Microbiol. 2007;123:26–33. PubMed

Massei G., Kindberg J., Licoppe A., Gačić D., Šprem N., Kamler J., Baubet E., Hohmann U., Monaco A., Ozolinš J., Cellina S., Podgórski T., Fronseca C., Markov N., Pokorny B., Rosell C., Náhlik A. Wild boar populations up, numbers of hunters down? A review of trends and implications for Europe. Pest Manag. Sci. 2015;71:492–500. PubMed

Matthijnssens J., Ciarlet M., Rahman M., Attoui H., Banyai K., Estes M.K., Gentsch J.R., Iturriza-Gomara M., Kirkwood C.D., Martella V., Mertens P.P., Nakagomi O., Patton J.T., Ruggeri F.M., Saif L.J., Santos N., Steyer A., Taniguchi K., Desselberger U., Van Ranst M. Recommendations for the calssification of group A rotaviruses using all 11 genomic RNA segments. Arch. Virol. 2008;153:1621–1629. PubMed PMC

Matthijnssens J., Rahman M., Ciarlet M., Zeller M., Heylen E., Nakagomi T., Uchida R., Hassan Z., Azim T., Nakagomi O., Van Ranst M. Reassortment of human rotavirus gene segments into G11 rotavirus strains. Emerg. Infect. Dis. 2010;16:625–630. PubMed PMC

McGregor G.F., Gottschalk M., Godson D.L., Wilkins W., Bollinger T.K. Disease risk associated with free-ranging wild boar in Saskatchewan. Can. Vet. J. 2015;56:838–844. PubMed PMC

Meier R.K., Ruiz-Fons F., Ryser-Degiorgis M.-P. A picture of trends in Aujeszky’s disease virus exposure in wild boar in the Swiss and European contexts. BMC Vet. Res. 2015;11:277. PubMed PMC

Meng X.-J., Lindsay D.S., Sriranganathan N. Wild boars as sources for infectious diseases in livestock and human. Phil. Trans. R. Soc. B. 2009;364:2697–2707. PubMed PMC

Moenning V. The control of classical swine fever in wild boar. Front. Microbiol. 2015;6:1211. PubMed PMC

Mouchantat S., Wernike K., Lutz W., Hoffmann B., Ulrich R.G., Börner K., Wittstatt U., Beer M. A broad spectrum screening of Schmallenberg virus antibodies in wildlife animals in Germany. Vet. Res. 2015;46:99. doi: 10.1186/s13567-015-0232-x. PubMed DOI PMC

Moutelíková R., Prodělalová J., Dufková L. Prevalence study and phylogenetic analysis of group C porcine rotavirus in the Czech Republic revealed a high level of VP6 gene heterogeneity within porcine cluster I1. Arch. Virol. 2014;159:1163–1167. PubMed

Mullick S., Mukherjee A., Ghosh S., Pazhani G.P., Sur D., Manna B., Nataro J.P., Levine M.M., Ramamurthy T., Chawla-Sarkar M. Genomic analysis of human rotavirus strains G6P[14] and G11P[25] isolated from Kolkata in 2009 reveals interspecies transmission and complex reassortment events. Infect. Genet. Evol. 2013;14:15–21. PubMed

Okadera K., Abe M., Ito N., Morikawa S., Yamasaki A., Masatani T., Nakagava K., Yamaoka S., Sugyiama M. Evidence of natural transmission of group A rotavirus between domestic pigs and wild boars (Sus scrofa) in Japan. Infect. Genet. Evol. 2013;20:54–60. PubMed

Papp H., Borzák R., Farkas S., Kisfali P., Lengyel G., Molnár P., Melegh B., Matthijnssens J., Martella V., Bányai K. Zoonotic transmission of reassortant porcine G4P[6] rotaviruses in Hungarian pediatric patients identified sporadically over a 15 year period. Infect. Genet. Evol. 2013;19:71–80. PubMed

Podgórski T., Lusseau D., Scandura M., Sönnichsen L., Jędrzejewska B. Long-lasting, kin-directed female interactions in a spatially structured wild boar social network. PLoS One. 2014;9:e99875. doi: 10.1371/journal.pone.0099875. PubMed DOI PMC

Prodělalová J. The survey of porcine teschoviruses, sapeloviruses and enteroviruses B infecting domestic pigs and wild boars in the Czech Republic between 2005 and 2011. Infect. Genet. Evol. 2012;12:1447–1451. PubMed

Ramírez E., Moreno V., Díaz N., Osorio F., Ruiz A., Neira V., Quezada M. Evaluation of the pathogenicity and transmissibility of a Chilean isolate of porcine reproductive and respiratory syndrome virus. Transbound. Emerg. Dis. 2008;55:115–124. PubMed

Reiner G., Fresen C., Bronnert S., Willems H. Porcine reproductive and respiratory syndrome virus (PRRSV) infection in wild boars. Vet. Microbiol. 2009;136:250–258. PubMed

Schlosser J., Vina-Rodriguez A., Fast C., Groschup M.H. Chronically infected wild boar can transmit genotype 3 hepatitis virus to domestic pigs. Vet. Microbiol. 2015;180:15–21. PubMed

Škrkal J., Rulík P., Fantínová K., Mihalík J., Timková J. Radiocaesium levels in game in the Czech Republic. J. Environ. Rad. 2015;139:18–23. PubMed

Sliz I., Vlasakova M., Jackova A., Vilcek S. Characterization of porcine parvovirus type 3 and porcine circovirus type 2 in wild boars (Sus scrofa) in Slovakia. J. Wildl. Dis. 2015;51:703–711. PubMed

Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 2013;30:2725–2729. PubMed PMC

Touloudi A., Valiakos G., Athanasiou L.V., Birtsas P., Giannakopulous A., Papaspyropoulos K., Kalaitzis C., Sokos C., Tsokana C.N., Spyrou V., Petrovska L., Billinis C. A serosurvey for selected pathogens in Greek European wild boar. Vet. Rec. Open. 2015;2:e000077. doi: 10.1136/vetreco-2014-000077. PubMed DOI PMC

Vengust G., Valenčak Z., Bidovec A. A serological survey of selected pathogens in wild boar in Slovenia. J. Vet. Med. B. 2006;53:24–27. PubMed PMC

Vilcek S., Molnar L., Vlasakova M., Jackova A. The first detection of PRRSV in wild boars in Slovakia. Berl. Münch. Tierärztl. Wochenschr. 2015;128:31–35. PubMed

Woźniakowski G., Kozak E., Kowalczyk A., Łyjak M., Pomorska-Mól M., Niemczuk K., Pejsak Z. Current status of African swine fever virus in a population of wild boar in eastern Poland 2014–2015. Arch. Virol. 2015 doi: 10.1007/s00705-015-2650-5. PubMed DOI PMC

Zeller M., Heylen E., De Coster S., Van Ranst M., Matthijnssens J. Full genome characterization of a porcine-like human G9P [6] rotavirus strain isolated from an infant in Belgium. Infect. Genet. Evol. 2012;12:1492–1500. PubMed

Zhou X., Wang Y.H., Ghosh S., Tang W.F., Pang B.B., Liu M.Q., Peng J.S., Zhou D.J., Kobayashi N. Genomic characterization of G3P[6], G4P[6] and G4P[8] human rotaviruses from Wuhan, China: evidence for interspecies transmission and reassortment events. Infect. Genet. Evol. 2015;33:55–71. PubMed