This study aimed to detect virulence factors, pathovars, and phylogenetic groups of Escherichia coli strains obtained from feces of calves with and without diarrhea up to 70 days old and to determine the association between occurrence of diarrhea, phylogenetic groups, and pathovars. Phylo-typing analysis of the 336 E. coli strains isolated from calves with Clermont method showed that 21 (6.25 %) belong to phylogroup A, 228 (67.85 %) to phylogroup B1, 2 (0.6 %) to phylogroup B2, 5 (1.49 %) to phylogroup C, 57 (16.96 %) to phylogroup E, and 3 (0.9 %) to phylogroup F. Phylogroup D was not identified and 20 strains (5.95 %) were assigned as "unknown." The distribution of phylogenetic groups among pathovars showed that NTEC belong to phylogroups B1 (17) and C (4); EPEC to phylogroups B1 (6) and E (8); STEC to phylogroups A (5), B1 (56), B2 (2), C (1), and E (15); EHEC to phylogroups B1 (95) and E (5); and ETEC to phylogroups A (3), B1 (7), and E (10). The EAST-1 strains were phylogroups A (13), B1 (47), E (19), and F (3); E. coli strains of "unknown" phylogroups belonged to pathovars EPEC (1), EHEC (2), STEC (7), and EAST-1 strains (6). ETEC was associated with diarrhea (P = 0.002). Our study did not find association between the phylogenetic background and occurrence of diarrhea (P = 0.164) but did find some relationship in phylogenetic group and pathovar. The study showed that EHEC and STEC are classified as phylogroup B1, EAST-1 phylogroup A, ETEC, and EPEC phylogroup E.

Departamento de Medicina Veterinária Preventiva Faculdade de Medicina Veterinária e Saúde Animal Universidade de São Paulo Av Prof Orlando Marques de Paiva 87 São Paulo São Paulo CEP 05508 270 Brazil

Escola de Veterinária Departamento de Medicina Veterinária Preventiva Universidade Federal de Minas Gerais UFMG Av Antônio Carlos 6627 Belo Horizonte Minas Gerais CEP30123 970 Brazil

Zobrazit více v PubMed

Vet Microbiol. 2005 Sep 30;110(1-2):77-85 PubMed

J Vet Sci. 2011 Jun;12(2):159-64 PubMed

Vet Microbiol. 2008 Sep 18;131(1-2):173-84 PubMed

Environ Microbiol Rep. 2013 Feb;5(1):58-65 PubMed

Prev Vet Med. 2010 Feb 1;93(2-3):162-9 PubMed

Vet Res. 2006 Jan-Feb;37(1):3-13 PubMed

Infect Immun. 2001 Nov;69(11):6999-7009 PubMed

J Clin Microbiol. 1995 May;33(5):1375-7 PubMed

Vet Microbiol. 2010 Jul 14;143(2-4):307-13 PubMed

Vet Res Commun. 2008 Aug;32(6):433-7 PubMed

Vet Res. 2010 Sep-Oct;41(5):57 PubMed

Vet Rec. 2010 Nov 27;167(22):858-61 PubMed

Front Cell Infect Microbiol. 2015 Mar 12;5:23 PubMed

Trop Anim Health Prod. 2012 Oct;44(7):1783-90 PubMed

Biomed Res Int. 2015;2015:846219 PubMed

J Clin Microbiol. 2003 Apr;41(4):1775-8 PubMed

Int J Food Microbiol. 2008 Sep 30;127(1-2):139-46 PubMed

Trop Anim Health Prod. 2015 Jan;47(1):3-11 PubMed

Mol Biol Evol. 2004 Jun;21(6):1085-94 PubMed

J Clin Microbiol. 2004 Nov;42(11):4937-46 PubMed

Clin Microbiol Rev. 2013 Oct;26(4):822-80 PubMed

Vet Clin North Am Food Anim Pract. 2010 Mar;26(1):29-56, table of contents PubMed

FEMS Microbiol Lett. 2006 Mar;256(2):251-7 PubMed

ScientificWorldJournal. 2015;2015:610403 PubMed

Infect Immun. 1995 Mar;63(3):1055-61 PubMed

ScientificWorldJournal. 2015;2015:258424 PubMed

Virulence. 2013 Jul 1;4(5):366-72 PubMed

J Clin Microbiol. 1997 Jan;35(1):223-7 PubMed

Vet Microbiol. 2005 Mar 20;106(1-2):87-95 PubMed

Vet Microbiol. 2001 Aug 20;81(4):315-29 PubMed

Water Res. 2016 Jan 1;88:30-38 PubMed

Int J Food Microbiol. 2007 Apr 20;115(3):297-306 PubMed

Infect Genet Evol. 2011 Apr;11(3):654-62 PubMed

Res Vet Sci. 2012 Jun;92(3):372-7 PubMed

Int J Food Microbiol. 2011 Mar 15;146(1):99-104 PubMed

Nat Rev Microbiol. 2010 Mar;8(3):207-17 PubMed

J Clin Microbiol. 1996 Dec;34(12):2921-8 PubMed

Microbiol Immunol. 2010 Jun;54(6):320-9 PubMed

Appl Environ Microbiol. 2014 Dec;80(23):7230-4 PubMed

Appl Environ Microbiol. 1996 Sep;62(9):3350-4 PubMed

Distribution of pathogenicity island markers and virulence factors in new phylogenetic groups of uropathogenic Escherichia coli isolates