Transcriptional analysis of genes encoding β-glucosidase of Schizophyllum commune KUC9397 under optimal conditions
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27905050
DOI
10.1007/s12223-016-0484-5
PII: 10.1007/s12223-016-0484-5
Knihovny.cz E-zdroje
- MeSH
- aktivace transkripce MeSH
- beta-glukosidasa biosyntéza genetika MeSH
- kultivační média chemie MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- molekulární sekvence - údaje MeSH
- Schizophyllum enzymologie genetika růst a vývoj MeSH
- sekvence aminokyselin MeSH
- sekvenční analýza DNA MeSH
- sekvenční homologie aminokyselin MeSH
- stanovení celkové genové exprese * MeSH
- upregulace MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- beta-glukosidasa MeSH
- kultivační média MeSH
The present study was conducted to determine the gene responsible for beta-glucosidase (BGL) production and to generate a full-length complementary DNA (cDNA) of one of the putative BGL genes, which showed a significant expression level when Schizophyllum commune KUC9397 was grown in optimized medium. The relative expression levels of seven genes encoding BGL of S. commune KUC9397 were determined with real-time quantitative reverse transcription PCR in cellulose-containing optimized medium (OM) compared to glucose-containing basal medium (BM). The most abundant transcript was bgl3a in OM. The transcript number of the bgl3a increased more than 57.60-fold when S. commune KUC9397 was grown on cellulose-containing OM compared to that on glucose-containing BM. The bgl3a was identified, and a deduced amino acid sequence of bgl3a shared homology (97%) with GH3 BGL of S. commune H4-8. This is the first report showing the transcription levels of genes encoding BGL and identification of full-length cDNA of glycoside hydrolase 3 (GH3) BGL from S. commune. Furthermore, this study is one of the steps for consolidated bioprocessing of lignocellulosic biomass to bioethanol.
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