Distribution of local anesthetics between aqueous and liposome phases
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27955893
DOI
10.1016/j.chroma.2016.12.005
PII: S0021-9673(16)31617-X
Knihovny.cz E-zdroje
- Klíčová slova
- Cholesterol, Distribution constants, EOF markers, Liposome electrokinetic chromatography, Local anesthetics, Red blood cell lipids,
- MeSH
- anestetika lokální chemie MeSH
- chromatografie micelární elektrokinetická kapilární * MeSH
- erytrocyty metabolismus MeSH
- fosfatidylcholiny chemie MeSH
- fosfolipidy chemie izolace a purifikace MeSH
- lidé MeSH
- lidokain chemie MeSH
- lipidové dvojvrstvy chemie MeSH
- liposomy chemie MeSH
- teplota MeSH
- voda chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- anestetika lokální MeSH
- fosfatidylcholiny MeSH
- fosfolipidy MeSH
- lidokain MeSH
- lipidové dvojvrstvy MeSH
- liposomy MeSH
- voda MeSH
Liposomes were used as biomimetic models in capillary electrokinetic chromatography (EKC) for the determination of distribution constants (KD) of certain local anesthetics and a commonly used preservative. Synthetic liposomes comprised phosphatidylcholine and phosphatidylglycerol phospholipids with and without cholesterol. In addition, ghost liposomes made from red blood cell (RBC) lipid extracts were used as pseudostationary phase to acquire information on how the liposome composition affects the interactions between anesthetics and liposomes. These results were compared with theoretical distribution coefficients at pH 7.4. In addition to 25°C, the distribution constants were determined at 37 and 42°C to simulate physiological conditions. Moreover, the usability of five electroosmotic flow markers in liposome (LEKC) and micellar EKC (MEKC) was studied. LEKC was proven to be a convenient and fast technique for obtaining data about the distribution constants of local anesthetics between liposome and aqueous phase. RBC liposomes can be utilized for more representative model of cellular membranes, and the results indicate that the distribution constants of the anesthetics are greatly dependent on the used liposome composition and the amount of cholesterol, while the effect of temperature on the distribution constants is less significant.
Department of Chemistry POB 55 00014 University of Helsinki Finland
Department of Environmental Science and Analytical Chemistry Stockholm University Sweden
Helsinki Eye Lab Ophthalmology University of Helsinki and Helsinki University Hospital Finland
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