New silver complexes with bioactive glycine and nicotinamide molecules - Characterization, DNA binding, antimicrobial and anticancer evaluation
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
27997856
DOI
10.1016/j.jinorgbio.2016.12.003
PII: S0162-0134(16)30496-2
Knihovny.cz E-resources
- Keywords
- Antimicrobial effect, Cytotoxicity, Intercalation, Silver complex, Stability, Topo I and II inhibition assay,
- MeSH
- Enzyme Activation drug effects MeSH
- Anti-Infective Agents chemistry pharmacology MeSH
- Bacteria drug effects MeSH
- DNA Topoisomerases metabolism MeSH
- DNA chemistry metabolism MeSH
- Glycine chemistry MeSH
- Inhibitory Concentration 50 MeSH
- Coordination Complexes chemistry pharmacology MeSH
- Crystallography, X-Ray MeSH
- Mice MeSH
- Cell Line, Tumor MeSH
- Niacinamide chemistry MeSH
- Antineoplastic Agents chemistry pharmacology MeSH
- Silver chemistry pharmacology MeSH
- Cell Survival drug effects MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anti-Infective Agents MeSH
- DNA Topoisomerases MeSH
- DNA MeSH
- Glycine MeSH
- Coordination Complexes MeSH
- Niacinamide MeSH
- Antineoplastic Agents MeSH
- Silver MeSH
This study introduces a pair of newly synthesized silver complexes, [Ag2(HGly)2]n(NO3)2n (1) and [Ag(Nam)2]NO3·H2O (2) (Gly - glycine, Nam - nicotinamide), that were prepared and characterized by relevant methods in solid state (elemental, spectral, thermal and structural analysis) and their stability in solution was verified by 1H NMR measurements. Moreover, suitable reaction conditions were observed by potentiometry depending on pH in case of binary system Ag-Gly. X-ray analysis confirmed argentophilic interactions in complex 1 with an Ag1-Ag2 distance of 2.8018(6) Å. Antimicrobial testing indicates higher growth inhibition effect of complex 1 than complex 2. Moreover the effectivity of both complexes against bacteria (Staphylococcus aureus and Escherichia coli) is superior (or similar) to that of the commercially available Ag(I) sulfadiazine, AgSD (used, for example, in Dermazine cream). The binding of the Ag(I) complexes to calf thymus DNA was investigated using electronic absorption, fluorescence and circular dichroism spectrophotometry. The Stern-Volmer quenching constants obtained from the linear quenching plot were estimated in the range from 2.01×103 to 20.34×103M-1. The results of topoisomerase I and topoisomerase II (Topo I and Topo II) inhibition assay suggested that complex 2 inhibits the enzyme activity of both enzymes at a concentration of 2μM. The cytotoxicity of both complexes on L1210 leukemia cells was revealed to be approximately three times higher than that of cisplatin. Moreover, the new Ag(I) complexes also induced apoptosis of the leukemia cells. The high DNA binding activity of these complexes is considered to be responsible for their cytotoxic effects.
References provided by Crossref.org
Antimicrobial and Anticancer Application of Silver(I) Dipeptide Complexes
