New silver complexes with bioactive glycine and nicotinamide molecules - Characterization, DNA binding, antimicrobial and anticancer evaluation
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
27997856
DOI
10.1016/j.jinorgbio.2016.12.003
PII: S0162-0134(16)30496-2
Knihovny.cz E-zdroje
- Klíčová slova
- Antimicrobial effect, Cytotoxicity, Intercalation, Silver complex, Stability, Topo I and II inhibition assay,
- MeSH
- aktivace enzymů účinky léků MeSH
- antiinfekční látky chemie farmakologie MeSH
- Bacteria účinky léků MeSH
- DNA-topoisomerasy metabolismus MeSH
- DNA chemie metabolismus MeSH
- glycin chemie MeSH
- inhibiční koncentrace 50 MeSH
- komplexní sloučeniny chemie farmakologie MeSH
- krystalografie rentgenová MeSH
- myši MeSH
- nádorové buněčné linie MeSH
- niacinamid chemie MeSH
- protinádorové látky chemie farmakologie MeSH
- stříbro chemie farmakologie MeSH
- viabilita buněk účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antiinfekční látky MeSH
- DNA-topoisomerasy MeSH
- DNA MeSH
- glycin MeSH
- komplexní sloučeniny MeSH
- niacinamid MeSH
- protinádorové látky MeSH
- stříbro MeSH
This study introduces a pair of newly synthesized silver complexes, [Ag2(HGly)2]n(NO3)2n (1) and [Ag(Nam)2]NO3·H2O (2) (Gly - glycine, Nam - nicotinamide), that were prepared and characterized by relevant methods in solid state (elemental, spectral, thermal and structural analysis) and their stability in solution was verified by 1H NMR measurements. Moreover, suitable reaction conditions were observed by potentiometry depending on pH in case of binary system Ag-Gly. X-ray analysis confirmed argentophilic interactions in complex 1 with an Ag1-Ag2 distance of 2.8018(6) Å. Antimicrobial testing indicates higher growth inhibition effect of complex 1 than complex 2. Moreover the effectivity of both complexes against bacteria (Staphylococcus aureus and Escherichia coli) is superior (or similar) to that of the commercially available Ag(I) sulfadiazine, AgSD (used, for example, in Dermazine cream). The binding of the Ag(I) complexes to calf thymus DNA was investigated using electronic absorption, fluorescence and circular dichroism spectrophotometry. The Stern-Volmer quenching constants obtained from the linear quenching plot were estimated in the range from 2.01×103 to 20.34×103M-1. The results of topoisomerase I and topoisomerase II (Topo I and Topo II) inhibition assay suggested that complex 2 inhibits the enzyme activity of both enzymes at a concentration of 2μM. The cytotoxicity of both complexes on L1210 leukemia cells was revealed to be approximately three times higher than that of cisplatin. Moreover, the new Ag(I) complexes also induced apoptosis of the leukemia cells. The high DNA binding activity of these complexes is considered to be responsible for their cytotoxic effects.
Citace poskytuje Crossref.org
Antimicrobial and Anticancer Application of Silver(I) Dipeptide Complexes
