Toward ideal separation by size-exclusion chromatography
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
28139225
DOI
10.1016/j.chroma.2017.01.038
PII: S0021-9673(17)30106-1
Knihovny.cz E-resources
- Keywords
- Band broadening function, Diffusion, Excess kurtosis, Separation mechanism, Size-exclusion chromatography, Skew,
- MeSH
- Chemistry Techniques, Analytical methods MeSH
- Diffusion MeSH
- Chromatography, Gel * MeSH
- Molecular Weight MeSH
- Polymers chemistry isolation & purification MeSH
- Polystyrenes chemistry MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Polymers MeSH
- Polystyrenes MeSH
In analyses of polystyrene standards by size-exclusion chromatography for polymers of molecular weight about thirty thousand and higher, the separation improved with decreasing flow-rate is in accord with Giddings observation of transversal diffusion as a factor decreasing the broadening of the band broadening function. The variance of the elution curves is larger than it corresponds to dispersity M¯w/M¯n and the shape of the elution curves depends on the experimental conditions which suggests that it reflects properties of the band broadening function. The skew and the excess kurtosis of the elution curves increase near the exclusion limit in accord with theoretical prediction for the band broadening function.
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