The composition and biomechanical properties of human cryopreserved aortas, pulmonary trunks, and aortic and pulmonary cusps
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články
PubMed
28434910
DOI
10.1016/j.aanat.2017.03.004
PII: S0940-9602(17)30046-8
Knihovny.cz E-zdroje
- Klíčová slova
- Calcification, Elastin, Microcracks, Tissue banking, Ultimate strain, Wall thickness, Young’s modulus,
- MeSH
- alografty MeSH
- aorta anatomie a histologie fyziologie MeSH
- aortální chlopeň anatomie a histologie fyziologie transplantace MeSH
- arteria pulmonalis anatomie a histologie fyziologie MeSH
- biomechanika fyziologie MeSH
- kryoprezervace MeSH
- lidé MeSH
- mechanický stres MeSH
- pevnost v tahu MeSH
- plicní chlopeň anatomie a histologie fyziologie transplantace MeSH
- pružnost MeSH
- tkáňové banky MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
Human cryopreserved allografts of pulmonary and aortic heart valves, aortas and pulmonary trunks are used for valve replacement. However, it is unknown how the composition of these allografts relate to their mechanical properties. Our aims were to correlate the histological compositions and passive mechanical properties of aortic and pulmonary valves and to observe the microcracks of aortas and pulmonary trunks. The following parameters were quantified: ultimate stress; ultimate strain; Young's modulus of elasticity; valve cusp wall thickness; pulmonary and aortic intima-media thickness; area fraction of elastin, collagen and calcification; and length density of elastic fibres. The propagation of experimentally induced microcracks avoided elastic fibres. Ultimate strain was negatively correlated with the area fraction of calcification (r=-0.4) in aortas. Ultimate stress (r=0.27) and Young's modulus in small deformation (r=0.29) and in large deformation (r=0.32) correlated with wall thickness in valve cusps. Young's modulus (r=0.34) and ultimate strain (r=0.31) correlated with intima-media thickness. Ultimate strain correlated with the area fraction of elastin (r=-0.40) and collagen in the arteries (r=0.31). As conventional histology does not fully explain the mechanical properties of cryopreserved grafts, both morphological and biomechanical tests should be used complementarily when characterizing the ageing of the grafts.
Citace poskytuje Crossref.org
The time has come to extend the expiration limit of cryopreserved allograft heart valves