Activity of cholinesterases in a young and healthy middle-European population: Relevance for toxicology, pharmacology and clinical praxis
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
28465191
DOI
10.1016/j.toxlet.2017.04.017
PII: S0378-4274(17)30167-4
Knihovny.cz E-zdroje
- Klíčová slova
- Acetylcholinesterase, Butyrylcholinesterase, Clinical study, Intra-population factors, Organophosphates,
- MeSH
- acetylcholinesterasa krev MeSH
- biologické markery krev MeSH
- butyrylcholinesterasa metabolismus MeSH
- dospělí MeSH
- GPI-vázané proteiny krev MeSH
- hemoglobiny analýza MeSH
- hromadné neštěstí MeSH
- hygiena práce MeSH
- kouření krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- otrava organofosfáty krev enzymologie MeSH
- referenční hodnoty MeSH
- sexuální faktory MeSH
- věkové faktory MeSH
- zdraví dobrovolníci pro lékařské studie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- acetylcholinesterasa MeSH
- ACHE protein, human MeSH Prohlížeč
- biologické markery MeSH
- butyrylcholinesterasa MeSH
- GPI-vázané proteiny MeSH
- hemoglobiny MeSH
The activity of human cholinesterases, erythrocyte acetylcholinesterase (AChE; EC 3.1.1.7) and plasma butyrylcholinesterase (BChE; EC 3.1.1.8) represents an important marker when monitoring exposure to pesticides/nerve agents, and may also be used in occupational medicine in diagnosis and prognosis of some diseases. In this study "normal/baseline" AChE and BChE activity has been investigated in a young and healthy population, with subsequent evaluation of several intra-population factors including sex, age (categories 18-25, 26-35 and 36-45 years old) and smoker status. The modified Ellman's method was used for enzyme activity assessment in 387 young and healthy individuals (201 males and 186 females aged 18-45). A significant inter-sexual difference in AChE and BChE activity was found (AChE: 351±67 for males and 377±65 for females, (μmol/min)/(μmol of hemoglobin), p<0.001; BChE: 140±33 for males and 109±29 for females, μkat/l, p<0.001; mean±SD). Despite the finding that mean AChE activity somewhat decreased whereas BChE activity grew within the age categories of the tested subjects, no significant effect of age on cholinesterase activity was found (p>0.05). Smoking influenced cholinesterase activity - AChE activity in smokers was elevated (approx. 3% in males; 8% in females) relative to that in non-smokers (p<0.05). Smoking was found not to have any effect on BChE activity. Reference values based on confidence intervals for AChE and BChE activity were established. The presented results might be useful in routine clinical practice where the monitoring of blood AChE and plasma BChE activity is crucial for prognosis and diagnosis of organophosphate poisoning, in occupational medicine and in relevant mass casualty scenarios.
Citace poskytuje Crossref.org
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