Activity of cholinesterases in a young and healthy middle-European population: Relevance for toxicology, pharmacology and clinical praxis
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
28465191
DOI
10.1016/j.toxlet.2017.04.017
PII: S0378-4274(17)30167-4
Knihovny.cz E-resources
- Keywords
- Acetylcholinesterase, Butyrylcholinesterase, Clinical study, Intra-population factors, Organophosphates,
- MeSH
- Acetylcholinesterase blood MeSH
- Biomarkers blood MeSH
- Butyrylcholinesterase metabolism MeSH
- Adult MeSH
- GPI-Linked Proteins blood MeSH
- Hemoglobins analysis MeSH
- Mass Casualty Incidents MeSH
- Occupational Health MeSH
- Smoking blood MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Organophosphate Poisoning blood enzymology MeSH
- Reference Values MeSH
- Sex Factors MeSH
- Age Factors MeSH
- Healthy Volunteers MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- Acetylcholinesterase MeSH
- ACHE protein, human MeSH Browser
- Biomarkers MeSH
- Butyrylcholinesterase MeSH
- GPI-Linked Proteins MeSH
- Hemoglobins MeSH
The activity of human cholinesterases, erythrocyte acetylcholinesterase (AChE; EC 3.1.1.7) and plasma butyrylcholinesterase (BChE; EC 3.1.1.8) represents an important marker when monitoring exposure to pesticides/nerve agents, and may also be used in occupational medicine in diagnosis and prognosis of some diseases. In this study "normal/baseline" AChE and BChE activity has been investigated in a young and healthy population, with subsequent evaluation of several intra-population factors including sex, age (categories 18-25, 26-35 and 36-45 years old) and smoker status. The modified Ellman's method was used for enzyme activity assessment in 387 young and healthy individuals (201 males and 186 females aged 18-45). A significant inter-sexual difference in AChE and BChE activity was found (AChE: 351±67 for males and 377±65 for females, (μmol/min)/(μmol of hemoglobin), p<0.001; BChE: 140±33 for males and 109±29 for females, μkat/l, p<0.001; mean±SD). Despite the finding that mean AChE activity somewhat decreased whereas BChE activity grew within the age categories of the tested subjects, no significant effect of age on cholinesterase activity was found (p>0.05). Smoking influenced cholinesterase activity - AChE activity in smokers was elevated (approx. 3% in males; 8% in females) relative to that in non-smokers (p<0.05). Smoking was found not to have any effect on BChE activity. Reference values based on confidence intervals for AChE and BChE activity were established. The presented results might be useful in routine clinical practice where the monitoring of blood AChE and plasma BChE activity is crucial for prognosis and diagnosis of organophosphate poisoning, in occupational medicine and in relevant mass casualty scenarios.
References provided by Crossref.org
Brominated oxime nucleophiles are efficiently reactivating cholinesterases inhibited by nerve agents
