Needleless emulsion electrospinning for the regulated delivery of susceptible proteins
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
28508471
DOI
10.1002/term.2474
Knihovny.cz E-resources
- Keywords
- Pluronic F-68, emulsion electrospinning, growth factor, needleless core/shell electrospinning, time-regulated release,
- MeSH
- Biocompatible Materials pharmacology MeSH
- Dextrans chemistry MeSH
- Emulsions chemistry MeSH
- Needles MeSH
- Collagen Type II metabolism MeSH
- Horseradish Peroxidase metabolism MeSH
- Mesenchymal Stem Cells cytology drug effects metabolism MeSH
- Intercellular Signaling Peptides and Proteins pharmacology MeSH
- Swine, Miniature MeSH
- Nanofibers chemistry ultrastructure MeSH
- Poloxamer chemistry MeSH
- Polyesters chemistry MeSH
- Swine MeSH
- Proteins administration & dosage MeSH
- Rhodamines chemistry MeSH
- Tissue Engineering methods MeSH
- Tissue Scaffolds chemistry MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biocompatible Materials MeSH
- Dextrans MeSH
- Emulsions MeSH
- Collagen Type II MeSH
- Horseradish Peroxidase MeSH
- Intercellular Signaling Peptides and Proteins MeSH
- Poloxamer MeSH
- polycaprolactone MeSH Browser
- Polyesters MeSH
- Proteins MeSH
- Rhodamines MeSH
- tetramethylrhodamine isothiocyanate MeSH Browser
In the present work, we developed a novel needleless emulsion electrospinning technique that improves the production rate of the core/shell production process. The nanofibres are based on poly-ε-caprolactone (PCL) as a continuous phase combined with a droplet phase based on Pluronic F-68 (PF-68). The PCL-PF-68 nanofibres show a time-regulated release of active molecules. Needleless emulsion electrospinning was used to encapsulate a diverse set of compounds to the core phase [i.e. 5-(4,6-dichlorotriazinyl) aminofluorescein -PF-68, horseradish peroxidase, Tetramethylrhodamine-dextran, insulin growth factor-I, transforming growth factor-β and basic fibroblast growth factor]. In addition, the PF-68 facilitates the preservation of the bioactivity of delivered proteins. The system's potential was highlighted by an improvement in the metabolic activity and proliferation of mesenchymal stem cells. The developed system has the potential to deliver susceptible molecules in tissue-engineering applications.
References provided by Crossref.org
Advances in Electrospun Hybrid Nanofibers for Biomedical Applications
