First Report and Comparative Study of Steinernema surkhetense (Rhabditida: Steinernematidae) and its Symbiont Bacteria from Subcontinental India
Status PubMed-not-MEDLINE Language English Country Poland Media print
Document type Journal Article
PubMed
28512381
PubMed Central
PMC5411258
DOI
10.21307/jofnem-2017-049
Knihovny.cz E-resources
- Keywords
- D2-D3 domain, ITS-rDNA, Xenorhabdus stockiae, entomopathogenic nematode, mt COI gene,
- Publication type
- Journal Article MeSH
Two populations (CS19 and CS20) of entomopathogenic nematodes were isolated from the soils of vegetable fields from Bijnor district, India. Based on morphological, morphometrical, and molecular studies, the nematodes were identified as Steinernema surkhetense. This work represents the first report of this species in India. The infective juveniles (IJs) showed morphometrical and morphological differences, with the original description based on longer IJs size. The IJs of the Indian isolates possess six ridges in their lateral field instead of eight reported in the original description. The analysis of ITS-rDNA sequences revealed nucleotide differences at 345, 608, and 920 positions in aligned data. No difference was observed in D2-D3 domain. The S. surkhetense COI gene was studied for the first time as well as the molecular characterization of their Xenorhabdus symbiont using the sequences of recA and gyrB genes revealing Xenorhabdus stockiae as its symbiont. These data, together with the finding of X. stockiae, suggest that this bacterium is widespread among South Asian nematodes from the "carpocapsae" group. Virulence of both isolates was tested on Spodoptera litura. The strain CS19 was capable to kill the larvae with 31.78 IJs at 72 hr, whereas CS20 needed 67.7 IJs.
Institute of Entomology Branišovská 31 37005 České Budějovice Czech Republic
Nematology Laboratory Department of Zoology Chaudhary Charan Singh University Meerut 250004 India
See more in PubMed
Adams BJ. Species concept and the evolutionary paradigm in modern nematology. Journal of Nematology. 1998;30:1–21. PubMed PMC
Akhurst RJ. Morphological and functional dimorphism in Xenorhabdus spp., bacteria symbiotically associated with the insect pathogenic nematodes Neoaplectana and Heterorhabditis. Journal of General Microbiology. 1980;121:303–309. PubMed
Alsaiyah MAM, Ebssa L, Zenner A, O’Callaghan KM, Griffin CT. Sex ratios and sex-biased infection behaviour in the entomopathogenic nematode genus Steinernema. International Journal for Parasitology. 2009;39:725–734. PubMed
Bedding RA, Akhurst RJ. A simple technique for the detection of insect parasitic nematodes in soil. Nematologica. 1975;21:109–110.
Bedding RA, Miller LA. Disinfecting black currant cuttings of Synanthedon tipuliformis, using the insect parasitic nematode, Neoaplectana bibionis. Environmental Entomology. 1981;10:449–453.
Campos-Herrera R, Escuer M, Robertson L, Gutiérrez C. Morphological and ecological characterization of Steinernema feltiae (Rhabditida: Steinernematidae) Rioja strain isolated from Bibio hortulanus (Diptera: Bibionidae) in Spain. Journal of Nematology. 2006;38:68–75. PubMed PMC
Castresana J. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution. 2000;17:540–552. PubMed
Chen S, Glazer I, Gollop N, Cash P, Argo E, Innes A, Stewart E, Davidson I, Wilson MJ. Proteomic analysis of the entomopathogenic nematode Steinernema feltiae IS-6 IJs under evaporative and osmotic stresses. Molecular and Biochemical Parasitology. 2009;145:195–204. PubMed
Courtney WD, Polley D, Miller VL. TAF, an improved fixative in nematode technique. Plant Disease Reporter. 1955;39:570–571.
Divya K, Sankar M. Entomopathogenic nematodes in pest management. Indian Journal of Science and Technology. 2009;2:53–60.
Gal TZ, Solomon A, Glazer I, Koltai H. Alterations in the levels of glycogen and glycogen synthase transcripts during desiccation in the insect-killing nematode Steinernema feltiae IS-6. Journal of Parasitology. 2001;87:725–732. PubMed
Ganguly S, Singh LK. Steinernema thermophilum (Rhabditida: Steinernematidae) from India. International Journal of Nematology. 2000;10:183–191.
Ganguly S, Singh LK. Report on pygmy females in Steinernema thermophilum Ganguly and Singh, 2000 (Rhabditida: Steinernematidae) Indian Journal of Nematology. 2003;33:195–196.
Ganguly S, Singh M, Lal M, Singh LK, Vyas RV, Patel DJ. New record of an entomopathogenic nematode, Steinernema riobrave Cabanillas, Poinar and Raulston, 1994 from Gujarat, India. Indian Journal of Nematology. 2002;32:223.
Harris NC, Coonan TJ, King JL, Dunn RR. Endemism in host–parasite interactions among island populations of an endangered species. Diversity and Distributions. 2013;19:377–38.
Hazir S, Stock SP, Keskin N. A new entomopathogenic nematode, Steinernema anatoliense n. sp. (Rhabditida: Steinernematidae), from Turkey. Systematic Parasitology. 2003;55:211–220. PubMed
Huelsenbeck JP, Ronquist F. MR-BAYES: Bayesian inference of phylogeny. Bioinformatics. 2001;17:754–755. PubMed
Hunt DJ. 2007. Overview of taxonomy and systematics. Pp. 27–57 in K. B. Nguyen, and D. J. Hunt, eds. Entomopathogenic nematodes: Systematics, phylogeny and bacterial symbionts. Nematology Monographs and Perspectives vol. 5. Leiden, the Netherlands: Brill.
Hussaini SS, Ansari MA, Ahmad W, Subbotin SA. Identification of some Indian populations of Steinernema species (Nematoda) by RFLP analysis of ITS region of rDNA. International Journal of Nematology. 2001;11:73–76.
Kaya HK, Aguillera MM, Alumai A, Choo HY, De la Torre M, Fodor A, Ganguly S, Hazir S, Lakatos T, Pye A. Status of entomopathogenic nematodes and their symbiotic bacteria from selected countries or regions of the world. Biological Control. 2006;38:134–155.
Kaya HK, Gaugler R. Entomopathogenic nematodes. Annual Review of Entomology. 1993;38:181–206.
Kadav A, Lalramliana Efficacy of indigenous entomopathogenic nematodes from Meghalaya, India against the larvae of taro leaf beetle, Aplosonyx chalybaeus (Hope) Journal of Parasitic Diseases. 2012;36:149–154. PubMed PMC
Khatri-Chhetri HB, Waeyenberge L, Spiridonov S, Manandhar HK, Moens M. Two new species of Steinernema Travassos, 1927 with short infective juveniles from Nepal. Russian Journal of Nematology. 2011;19:53–74.
Lewis EE, Gaugler R, Harrison A. Entomopathogenic nematode host-finding: Response to contact cues by cruise and ambush foragers. Parasitology. 1992;105:309–315.
Lewis EE, Gaugler R, Harrison R. Response of cruiser and ambusher entomopathogenic nematodes (Steinernematidae) to host volatile cues. Canadian Journal of Zoology. 1993;71:765–769.
Maneesakorn P, Grewal PS, Chandrapatya A. Steinernema minutum sp. nov. (Rhabditida: Steinernema): A new entomopathogenic nematode from Thailand. International Journal of Nematology. (2010);20:27–42.
Nguyen KB, Smart GC. Scanning electron microscope studies of Steinernema glaseri (Nematoda: Steinernematidae) Nematologica. 1995;41:183–190.
Nguyen KB, Smart GC. Scanning electron microscope studies of spicules and gubernacula of Steinernema spp. (Nemata: Steinernematidae) Nematologica. 1997;43:465–480.
Nylander JAA. 2004. MrModeltest 2.0. Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Sweden.
Phan KL, Mráček Z, Půža V, Nermut J, Jarošová A. Steinernema huense sp. n., a new entomopathogenic nematode (Nematoda: Steinernematidae) from Vietnam. Nematology. 2014;16:761–775.
Poinar GO., Jr Description and biology of a new insect parasitic rhabditoid, Heterorhabditis bacteriophora n. gen. n. sp. ((Rhabditida: Heterorhabditidae fam.) Nematologica. 1976;21:463–470.
Půža V, Nermut’ J, Mráček Z, Gengler S, Haukeland S. Steinernema pwaniensis n. sp., a new entomopathogenic nematode (Nematoda: Steinernematidae) from Tanzania. Journal of Helminthology. 2017;91:20–34. PubMed
Rai M, Pandey AK. Towards a rainbow revolution. The Hindu Survey of Indian Agriculture, Sec. 2007;5:112–114.
San-Blas E. Progress on entomopathogenic nematology research: A bibliometric study of the last three decades: 1980-2010. Biological Control. 2013;66:102–124.
San-Blas E, Morales-Montero P, Portillo E, Nermut’ J, Půža V. Steinernema goweni n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Zulia State, Venezuela. Zootaxa. 2016;4067:200–214. PubMed
Sandström JP, Russel JA, White JP, Moran NA. Independent origins and horizontal transfer of bacterial symbionts of aphids. Molecular Ecology. 2001;10:217–228. PubMed
Seinhorst JW. A rapid method for the transfer of nematodes from fixative to anhydrous glycerine. Nematologica. 1959;4:67–69.
Shapiro-Ilan DI, Stuart DI, McCoy CW. Comparison of beneficial traits among strains of the entomopathogenic nematode, Steinernema carpocapsae, for control of Curculio caryae (Coleoptera: Curculionidae) Biological Control. 2003;28:129–136.
Stock SP, Somsook V, Reid A. Steinernema siamkayai n. sp. (Rhabditida: Steinernematidae), an entomopathogenic nematode from Thailand. Systematic Parasitology. 1998;41:105–113.
Tailliez P, Laroui C, Ginibre N, Paule A, Pagès S, Boemare N. Phylogeny of Photorhabdus and Xenorhabdus based on universally conserved protein-coding sequences and implications for the taxonomy of these two genera. Proposal of new taxa: X. vietnamensis sp. nov., P. luminescens subsp. caribbeanensis subsp. nov., P. luminescens subsp. hainanensis subsp. nov., P. temperata subsp. khanii subsp. nov., P. temperata subsp. tasmaniensis subsp. nov., and the reclassification of P. luminescens subsp. thracensis as P. temperata subsp. thracensis comb. nov. International Journal of Systematic and Evolutionary Microbiology. 2010;60:1921–1937. PubMed
Tailliez P, Pages S, Ginibre N, Boemare N. New insight into diversity in the genus Xenorhabdus, including the description of ten novel species. International Journal of Systematic and Evolutionary Microbiology. 2006;56:2805–2818. PubMed
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution. 2013;30:2725–2729. PubMed PMC
Travassos L. Sobre o genero Oxysomatium. Boletim Biologico, Sao Paulo. 1927;5:20–21.
White GF. A method for obtaining infective nematode larvae from cultures. Science. 1927;66:302–303. PubMed