Monochromatic green light induces an aberrant accumulation of geranylgeranyled chlorophylls in plants
Jazyk angličtina Země Francie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
28527413
DOI
10.1016/j.plaphy.2017.05.002
PII: S0981-9428(17)30150-X
Knihovny.cz E-zdroje
- Klíčová slova
- Chlorophyll, Chlorophyllide, Geranylgeranyl reductase, LIL3, Light quality, Phytyl chain,
- MeSH
- chlorofyl a MeSH
- chlorofyl metabolismus MeSH
- fotosyntéza fyziologie účinky záření MeSH
- hmotnostní spektrometrie MeSH
- oxidoreduktasy metabolismus MeSH
- světlo * MeSH
- světlosběrné proteinové komplexy metabolismus účinky záření MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chlorofyl a MeSH
- chlorofyl MeSH
- chlorophyll b MeSH Prohlížeč
- geranylgeranyl reductase MeSH Prohlížeč
- oxidoreduktasy MeSH
- světlosběrné proteinové komplexy MeSH
Light quality is an important environmental factor affecting the biosynthesis of photosynthetic pigments whose production seems to be affected not only quantitatively but also qualitatively. In this work, we set out to identify unusual pigment detected in leaves of barley (Hordeum vulgare L.) and explain its presence in plants grown under monochromatic green light (GL; 500-590 nm). The chromatographic analysis (HPLC-DAD) revealed that a peak belonging to this unknown pigment is eluted between chlorophyll (Chl) a and b. This pigment exhibited the same absorption spectrum and fluorescence excitation and emission spectra as Chl a. It was negligible in control plants cultivated under white light of the same irradiance (photosynthetic photon flux density of 240 μmol m-2 s-1). Mass spectrometry analysis of this pigment (ions m/z = 889 [M-H]-; m/z = 949 [M+acetic acid-H]-) indicates that it is Chl a with a tetrahydrogengeranylgeraniol side chain (containing two double bonds in a phytyl side chain; Chl aTHGG), which is an intermediate in Chl a synthesis. In plants grown under GL, the proportion of Chl aTHGG to total Chl content rose to approximately 8% and 16% after 7 and 14 days of cultivation, respectively. Surprisingly, plants cultivated under GL exhibited drastically increased concentration of the enzyme geranylgeranyl reductase, which is responsible for the reduction of phytyl chain double bonds in the Chl synthesis pathway. This indicates impaired activity of this enzyme in GL-grown plants. A similar effect of GL on Chl synthesis was observed for distinct higher plant species.
Centre Algatech Institute of Microbiology The Czech Academy of Sciences 379 81 Třeboň Czech Republic
Faculty of Science University of Ostrava 30 dubna 22 701 03 Ostrava 1 Czech Republic
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