Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modeling in Three Dimensions
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Bone model, Calcium phosphate foam, Cell culture, Hydroxyapatite, Osteoblast, Scaffold, Three-dimensional, Tissue engineering, Tricalcium phosphate,
- MeSH
- biologické modely * MeSH
- buněčné kultury MeSH
- buněčné mikroprostředí MeSH
- fosforečnany vápenaté chemie MeSH
- kosti a kostní tkáň cytologie MeSH
- krysa rodu Rattus MeSH
- poréznost MeSH
- testování materiálů MeSH
- tkáňové inženýrství metody MeSH
- tkáňové podpůrné struktury chemie MeSH
- velikost částic MeSH
- vyrobené materiály MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fosforečnany vápenaté MeSH
The present method describes the procedure to fabricate calcium phosphate foams with suitable open porosity, pore size, and composition to perform three-dimensional (3D) cell cultures with the objective to simulate the bone tissue microenvironment in vitro. Foams with two compositions but equivalent porosity can be fabricated. On the one hand, hydroxyapatite foams obtained by hydrolysis at 37 °C, with microstructure that mimics the small crystal size of the mineral component of bones, and on the other hand, beta tricalcium phosphate foams with polygonal grains obtained by sintering at 1100 °C. In the first part of the chapter the calcium phosphate foams are briefly described. Afterwards, the foaming process is described in detail, including alternatives to overcome processing problems than can arise. Finally, insights are provided on how to perform 3D cell cultures using the calcium phosphate foams as substrates.
Citace poskytuje Crossref.org
