Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modeling in Three Dimensions
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Bone model, Calcium phosphate foam, Cell culture, Hydroxyapatite, Osteoblast, Scaffold, Three-dimensional, Tissue engineering, Tricalcium phosphate,
- MeSH
- Models, Biological * MeSH
- Cell Culture Techniques MeSH
- Cellular Microenvironment MeSH
- Calcium Phosphates chemistry MeSH
- Bone and Bones cytology MeSH
- Rats MeSH
- Porosity MeSH
- Materials Testing MeSH
- Tissue Engineering methods MeSH
- Tissue Scaffolds chemistry MeSH
- Particle Size MeSH
- Manufactured Materials MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Calcium Phosphates MeSH
The present method describes the procedure to fabricate calcium phosphate foams with suitable open porosity, pore size, and composition to perform three-dimensional (3D) cell cultures with the objective to simulate the bone tissue microenvironment in vitro. Foams with two compositions but equivalent porosity can be fabricated. On the one hand, hydroxyapatite foams obtained by hydrolysis at 37 °C, with microstructure that mimics the small crystal size of the mineral component of bones, and on the other hand, beta tricalcium phosphate foams with polygonal grains obtained by sintering at 1100 °C. In the first part of the chapter the calcium phosphate foams are briefly described. Afterwards, the foaming process is described in detail, including alternatives to overcome processing problems than can arise. Finally, insights are provided on how to perform 3D cell cultures using the calcium phosphate foams as substrates.
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