The Impact of Hyperglycemia on VEGF Secretion in Retinal Endothelial Cells
Language English Country Bulgaria Media print
Document type Comparative Study, Journal Article
PubMed
28704181
DOI
10.1515/folmed-2017-0029
PII: /j/folmed.2017.59.issue-2/folmed-2017-0029/folmed-2017-0029.xml
Knihovny.cz E-resources
- Keywords
- VEGF, diabetic retinopathy, glucose, retina, retinal endothelial cell,
- MeSH
- Diabetic Retinopathy pathology physiopathology MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Endothelial Cells cytology MeSH
- Glucose metabolism pharmacology MeSH
- Haplorhini MeSH
- Hyperglycemia complications MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Cell Proliferation drug effects physiology MeSH
- Reference Values MeSH
- Retina cytology MeSH
- Sensitivity and Specificity MeSH
- Vascular Endothelial Growth Factor A analysis MeSH
- Cell Survival MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- Glucose MeSH
- Vascular Endothelial Growth Factor A MeSH
BACKGROUND: Diabetic retinopathy is a serious sight-threatening complication which is manifested by excessive angiogenesis in diabetic patients. AIM: We hypothesize that cultured Rhesus monkey retinal endothelial cells (RhRECs) respond to high glucose with a change in cell proliferation and vascular endothelial growth factor (VEGF) secretion. MATERIALS AND METHODS: In our study, 20 000 cells per well were treated without glucose or with 5.5 mM, 18.5 mM and 30 mM glucose for 24 hours. Viable cells were counted using trypan blue dye exclusion method. VEGF concentrations were measured in cell media by ELISA method. RESULTS: The number of viable cells incubated with 5.5 mM glucose increased significantly by 53.7% after 24 hours. In comparison, the number of viable cells decreased by 2.8% at 18.5 mM of glucose and by 20.4% at 30 mM of glucose after 24 hours of incubation. In contrast to this effect of glucose on the number of viable cells, a significant increase in VEGF levels (pg/mL) in the cell media with a glucose concentration of 0 mM compared to 5.5 mM of glucose was found. VEGF secretion in cell medium with 18.5 and 30 mM of glucose increased non-significantly in comparison with euglycemic levels. CONCLUSION: Our results show that viability of retinal endothelial cells and VEGF release are highly responsive to changes in glucose concentration. Such glucose-induced changes in retinal endothelial cells may negatively impact the integrity of the microvasculature in the diabetic retina leading to angiogenesis and microaneursym.
1st Department of Internal Medicine Faculty of Medicine Comenius University Bratislava Slovakia
Endocrinology Department Bogomolets National Medical University Kyiv Ukraine
Retina service Department of Ophthalmology King George’s Medical University Lucknow India
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