TRPV1 receptors contribute to paclitaxel-induced c-Fos expression in spinal cord dorsal horn neurons
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
28730839
DOI
10.33549/physiolres.933613
PII: 933613
Knihovny.cz E-resources
- MeSH
- Posterior Horn Cells drug effects metabolism MeSH
- Gene Expression MeSH
- Antineoplastic Agents, Phytogenic pharmacology MeSH
- TRPV Cation Channels antagonists & inhibitors physiology MeSH
- Rats MeSH
- Paclitaxel pharmacology MeSH
- Rats, Wistar MeSH
- Proto-Oncogene Proteins c-fos agonists biosynthesis genetics MeSH
- Spinal Cord Dorsal Horn drug effects metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antineoplastic Agents, Phytogenic MeSH
- TRPV Cation Channels MeSH
- Paclitaxel MeSH
- Proto-Oncogene Proteins c-fos MeSH
- Trpv1 protein, rat MeSH Browser
Transient receptor potential vanilloid type 1 (TRPV1) receptors are important in the development of different pathological chronic pain states. Here we examined the role of spinal cord TRPV1 receptors in the mechanisms leading to activation of dorsal horn neurons after paclitaxel (PAC) treatment. PAC is a widely used chemotherapeutic drug that often leads to development of painful neuropathy. Immunohistochemical analysis of c-Fos protein expression in dorsal horn neurons was used as a marker of neuronal activation. Rat spinal cord slices were processed for in vitro incubation with PAC (100 nM) and TRPV1 receptor antagonists (SB366791 and AMG9810; 10 microM). PAC treatment induced significant upregulation of c-Fos nuclear expression in superficial dorsal horn neurons that was diminished by TRPV1 receptor antagonists pre-incubation. These results further substantiated the role of spinal TRPV1 receptors in the development of paclitaxel-induced neuropathic pain and contribute to better understanding of the pathological mechanisms involved.
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