Identification and Characterization of Phospholipids with Very Long Chain Fatty Acids in Brewer's Yeast
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
P503 17-00027S
Grantová Agentura České Republiky
PubMed
28905226
DOI
10.1007/s11745-017-4294-6
PII: 10.1007/s11745-017-4294-6
Knihovny.cz E-zdroje
- Klíčová slova
- Negative electrospray ionization, Phospholipids, Very long chain fatty acids, Yeast,
- MeSH
- fosfolipidy analýza chemie MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací MeSH
- kyseliny mastné mononenasycené MeSH
- mastné kyseliny chemie MeSH
- molekulární struktura MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- Saccharomyces metabolismus MeSH
- tandemová hmotnostní spektrometrie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fosfolipidy MeSH
- kyseliny mastné mononenasycené MeSH
- mastné kyseliny MeSH
Yeast lipids and fatty acids (FA) were analyzed in Saccharomyces pastorianus from seven breweries and in the dietary yeast supplement Pangamin. GC-MS identified more than 30 FA, half of which were very-long chain fatty acids (VLCFA) with hydrocarbon chain lengths of ≥22 C atoms. Positional isomers ω-9 and ω-7 were identified in FA with C18-C28 even-numbered alkyl chains. The most abundant ω-7 isomer was cis-vaccenic acid. The structure of monounsaturated FA was proved by dimethyl disulfide adducts (position of double bonds and cis geometric configuration) and by GC-MS of pyridyl carbinol esters. Ultra-high performance liquid chromatography-tandem mass spectrometry with negative electrospray ionization identified the phospholipids phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine, with more than 150 molecular species. Wild-type unmutated brewer's yeast strains conventionally used for the manufacture of food supplements were found to contain VLCFA.
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