The paper deals with the possibilities of investigating the susceptibility to burnout syndrome in pharmacists and pharmaceutical assistants working in pharmacies. By using the Maslach Burnout Inventory, between November 2015 and January 2016, 53 healthcare professionals were contacted in 11 pharmacies (Brno, Vyškov). The results of the research show that with an increasing length of practice the average EE factor (emotional exhaustion) increases. The DP (depersonalization) factor does not increase significantly with the increase in the length of practice. Respondents with the longest experience are at high risk of burnout syndrome presented by the factor PA (personal satisfaction from work). It has also been shown that with an increasing age the factor EE slightly increases, the DP factor is in the low range, and the PA factor is significantly reduced in the "oldest" age category, it is high in the band.Key words: stress burnout syndrome pharmacists research.