Fab antibody fragment-functionalized liposomes for specific targeting of antigen-positive cells
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
28939491
DOI
10.1016/j.nano.2017.09.003
PII: S1549-9634(17)30170-3
Knihovny.cz E-zdroje
- Klíčová slova
- Active targeting, Antibody engineering, Immunoliposome, Liposome functionalization, Recombinant Fab antibody fragment,
- MeSH
- antigen CD48 metabolismus MeSH
- antigeny CD59 metabolismus MeSH
- imunoglobuliny - Fab fragmenty chemie imunologie metabolismus MeSH
- Jurkat buňky MeSH
- lidé MeSH
- liposomy chemie MeSH
- lymfom imunologie metabolismus patologie MeSH
- monoklonální protilátky chemie imunologie metabolismus MeSH
- myši MeSH
- nádorové buňky kultivované MeSH
- peptidové fragmenty imunologie metabolismus MeSH
- protein D asociovaný s plicním surfaktantem imunologie metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigen CD48 MeSH
- antigeny CD59 MeSH
- CD48 protein, human MeSH Prohlížeč
- CD59 protein, human MeSH Prohlížeč
- imunoglobuliny - Fab fragmenty MeSH
- liposomy MeSH
- monoklonální protilátky MeSH
- peptidové fragmenty MeSH
- protein D asociovaný s plicním surfaktantem MeSH
Liposomes functionalized with monoclonal antibodies or their antigen-binding fragments have attracted much attention as specific drug delivery devices for treatment of various diseases including cancer. The conjugation of antibodies to liposomes is usually achieved by covalent coupling using cross-linkers in a reaction that might adversely affect the characteristics of the final product. Here we present an alternative strategy for liposome functionalization: we created a recombinant Fab antibody fragment genetically fused on its C-terminus to the hydrophobic peptide derived from pulmonary surfactant protein D, which became inserted into the liposomal bilayer during liposomal preparation and anchored the Fab onto the liposome surface. The Fab-conjugated liposomes specifically recognized antigen-positive cells and efficiently delivered their cargo, the Alexa Fluor 647 dye, into target cells in vitro and in vivo. In conclusion, our approach offers the potential for straightforward development of nanomedicines functionalized with an antibody of choice without the need of harmful cross-linkers.
Centre of Biological Engineering University of Minho Campus of Gualtar Braga Portugal
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