Fab antibody fragment-functionalized liposomes for specific targeting of antigen-positive cells
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
28939491
DOI
10.1016/j.nano.2017.09.003
PII: S1549-9634(17)30170-3
Knihovny.cz E-resources
- Keywords
- Active targeting, Antibody engineering, Immunoliposome, Liposome functionalization, Recombinant Fab antibody fragment,
- MeSH
- CD48 Antigen metabolism MeSH
- CD59 Antigens metabolism MeSH
- Immunoglobulin Fab Fragments chemistry immunology metabolism MeSH
- Jurkat Cells MeSH
- Humans MeSH
- Liposomes chemistry MeSH
- Lymphoma immunology metabolism pathology MeSH
- Antibodies, Monoclonal chemistry immunology metabolism MeSH
- Mice MeSH
- Tumor Cells, Cultured MeSH
- Peptide Fragments immunology metabolism MeSH
- Pulmonary Surfactant-Associated Protein D immunology metabolism MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CD48 Antigen MeSH
- CD59 Antigens MeSH
- CD48 protein, human MeSH Browser
- CD59 protein, human MeSH Browser
- Immunoglobulin Fab Fragments MeSH
- Liposomes MeSH
- Antibodies, Monoclonal MeSH
- Peptide Fragments MeSH
- Pulmonary Surfactant-Associated Protein D MeSH
Liposomes functionalized with monoclonal antibodies or their antigen-binding fragments have attracted much attention as specific drug delivery devices for treatment of various diseases including cancer. The conjugation of antibodies to liposomes is usually achieved by covalent coupling using cross-linkers in a reaction that might adversely affect the characteristics of the final product. Here we present an alternative strategy for liposome functionalization: we created a recombinant Fab antibody fragment genetically fused on its C-terminus to the hydrophobic peptide derived from pulmonary surfactant protein D, which became inserted into the liposomal bilayer during liposomal preparation and anchored the Fab onto the liposome surface. The Fab-conjugated liposomes specifically recognized antigen-positive cells and efficiently delivered their cargo, the Alexa Fluor 647 dye, into target cells in vitro and in vivo. In conclusion, our approach offers the potential for straightforward development of nanomedicines functionalized with an antibody of choice without the need of harmful cross-linkers.
Centre of Biological Engineering University of Minho Campus of Gualtar Braga Portugal
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