Treatment of Vicia faba root tip cells with specific inhibitors to cyclin-dependent kinases leads to abnormal spindle formation
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- aparát dělícího vřeténka účinky léků MeSH
- buněčný cyklus účinky léků MeSH
- cyklin A genetika MeSH
- cyklin B genetika MeSH
- Fabaceae cytologie MeSH
- genetická transkripce MeSH
- inhibitory enzymů farmakologie MeSH
- kořeny rostlin cytologie účinky léků ultrastruktura MeSH
- léčivé rostliny * MeSH
- meristém cytologie účinky léků ultrastruktura MeSH
- mikrotubuly účinky léků ultrastruktura MeSH
- proteinkinasa CDC2 antagonisté a inhibitory genetika MeSH
- proteinkinasy závislé na vápníku a kalmodulinu metabolismus MeSH
- proteinkinasy metabolismus MeSH
- puriny farmakologie MeSH
- roskovitin MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bohemine MeSH Prohlížeč
- cyklin A MeSH
- cyklin B MeSH
- inhibitory enzymů MeSH
- proteinkinasa CDC2 MeSH
- proteinkinasy závislé na vápníku a kalmodulinu MeSH
- proteinkinasy MeSH
- puriny MeSH
- roskovitin MeSH
Many events during cell division are triggered by an evolutionary conserved regulator, the cyclin-dependent kinase (Cdk). Here we used two novel drugs, the purine analogues bohemine and roscovitine, to study the role of Cdks in cell cycle progression and microtubule organisation in Vicia faba root tip cells. Both drugs inhibited the activity of immunopurified Vicia faba and alfalfa Cdc2-kinase. The transcript levels of an A- and B-type cyclin, as well as of the cdc2 genes, declined in treated root tips, while the mRNA level of a D-type cyclin gene was not affected. An observed transient arrest at the G1/S and G2/M regulatory points indicated that inhibition of the Cdc2-kinase had an effect on both transitions. In contrast to the regular bipolar spindle in untreated cell, in drug-treated metaphase cells abnormally short and dense kinetochore microtubule fibres were observed. These microtubules were randomly arranged in the vicinity of the kinetochores and connected the chromosomes. Thus, the chromosomes were not aligned on the metaphase plate but were arranged in a circle, with kinetochores pointing inwards and chromosome arms pointing outwards. gamma-Tubulin, which plays a role in microtubule nucleation, also localised to the centre of the monopolar spindle. The observed abnormalities in mitosis, after inhibition of Cdc2-kinase by specific drugs, suggest a role for this enzyme in regulating some of the steps leading to a bipolar spindle structure.
Citace poskytuje Crossref.org
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