The microtubule cytoskeleton is critically important for spatio-temporal organization of eukaryotic cells. The nucleation of new microtubules is typically restricted to microtubule organizing centers (MTOCs) and requires γ-tubulin that assembles into multisubunit complexes of various sizes. γ-Tubulin ring complexes (TuRCs) are efficient microtubule nucleators and are associated with large number of targeting, activating and modulating proteins. γ-Tubulin-dependent nucleation of microtubules occurs both from canonical MTOCs, such as spindle pole bodies and centrosomes, and additional sites such as Golgi apparatus, nuclear envelope, plasma membrane-associated sites, chromatin and surface of pre-existing microtubules. Despite many advances in structure of γ-tubulin complexes and characterization of γTuRC interacting factors, regulatory mechanisms of microtubule nucleation are not fully understood. Here, we review recent work on the factors and regulatory mechanisms that are involved in centrosomal and non-centrosomal microtubule nucleation.

Department of Biology of Cytoskeleton Institute of Molecular Genetics Academy of Sciences of the Czech Republic Vídeňská 1083 142 20 Prague 4 Czech Republic

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Mol Biol Cell. 2008 Nov;19(11):4900-8 PubMed

Biol Open. 2013 Feb 15;2(2):238-50 PubMed

Nat Struct Mol Biol. 2015 Feb;22(2):132-7 PubMed

J Cell Biol. 2010 Dec 13;191(6):1089-95 PubMed

Yeast. 2006 Oct 15;23(13):1001-14 PubMed

Cell Cycle. 2007 Sep 1;6(17):2152-60 PubMed

Front Neurosci. 2015 Nov 10;9:431 PubMed

Nature. 1999 Jul 8;400(6740):178-81 PubMed

J Cell Biol. 2008 May 5;181(3):421-9 PubMed

J Cell Sci. 2000 Sep;113 ( Pt 17):3013-23 PubMed

J Cell Sci. 2009 Jul 1;122(Pt 13):2240-51 PubMed

PLoS One. 2011;6(11):e27423 PubMed

Int Immunol. 1999 Nov;11(11):1829-39 PubMed

Nat Cell Biol. 2005 Oct;7(10):961-8 PubMed

Cell Cycle. 2016 Jul 17;15(14 ):1844-54 PubMed

Nature. 2006 Mar 30;440(7084):697-701 PubMed

Biochem J. 2000 Mar 1;346 Pt 2:483-9 PubMed

Dev Cell. 2007 Jun;12(6):917-30 PubMed

Cell. 2013 Feb 14;152(4):768-77 PubMed

Mol Biol Cell. 2016 Mar 1;27(5):812-27 PubMed

Curr Biol. 2007 Nov 20;17(22):1960-6 PubMed

Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):6998-7003 PubMed

Nat Rev Mol Cell Biol. 2007 Feb;8(2):161-7 PubMed

EMBO J. 2003 Sep 15;22(18):4779-93 PubMed

J Immunol. 2006 Jun 15;176(12 ):7243-53 PubMed

J Neuropathol Exp Neurol. 2015 Jul;74(7):723-42 PubMed

Nat Cell Biol. 2015 Nov;17(11):1422-34 PubMed

EMBO J. 2009 Apr 22;28(8):1016-28 PubMed

Cell Motil Cytoskeleton. 1996;33(1):38-51 PubMed

J Cell Sci. 2009 Apr 15;122(Pt 8):1134-44 PubMed

PLoS One. 2012;7(1):e29919 PubMed

Curr Biol. 2015 Mar 30;25(7):R294-9 PubMed

Biochem J. 2002 Aug 1;365(Pt 3):889-95 PubMed

Curr Opin Cell Biol. 2006 Apr;18(2):179-84 PubMed

Nature. 1989 Apr 20;338(6217):662-4 PubMed

Biochem J. 2008 Dec 15;416(3):421-30 PubMed

Genome Biol Evol. 2014 Aug 27;6(9):2274-88 PubMed

Nat Cell Biol. 2012 Nov;14(11):1148-58 PubMed

Cell Cycle. 2013 Mar 15;12(6):899-906 PubMed

Plant Cell. 2003 Feb;15(2):465-80 PubMed

Mol Biol Cell. 2007 Mar;18(3):781-94 PubMed

J Cell Sci. 1998 Sep;111 ( Pt 18):2809-18 PubMed

EMBO J. 1996 Sep 2;15(17):4592-602 PubMed

Plant Cell. 2012 Apr;24(4):1494-509 PubMed

Plant Cell. 2000 Mar;12(3):433-42 PubMed

J Cell Sci. 2008 Dec 1;121(Pt 23):3971-80 PubMed

Mol Biol Cell. 2015 Sep 1;26(17):2957-62 PubMed

Nature. 2010 Aug 12;466(7308):879-82 PubMed

Nat Commun. 2013;4:1531 PubMed

Plant J. 2016 Jul;87(2):188-201 PubMed

J Cell Sci. 2005 Apr 15;118(Pt 8):1565-75 PubMed

Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11446-51 PubMed

Mol Biol Cell. 2016 Jul 15;27(14 ):2245-58 PubMed

Nat Struct Mol Biol. 2011 Jul 03;18(8):915-9 PubMed

Biochim Biophys Acta. 2016 Jun;1863(6 Pt A):1282-97 PubMed

J Immunol. 2015 May 1;194(9):4099-111 PubMed

J Cell Sci. 2012 Oct 1;125(Pt 19):4445-56 PubMed

Cell Motil Cytoskeleton. 2005 Jan;60(1):1-13 PubMed

Mol Biol Cell. 2004 Aug;15(8):3642-57 PubMed

Curr Biol. 2013 Jan 21;23(2):143-9 PubMed

J Cell Biol. 2005 Sep 26;170(7):1047-55 PubMed

J Cell Sci. 2006 Oct 15;119(Pt 20):4155-63 PubMed

J Biol Chem. 2014 Nov 14;289(46):31719-35 PubMed

PLoS One. 2014 Jul 08;9(7):e101471 PubMed

Plant J. 2007 Oct;52(2):322-31 PubMed

Elife. 2015 Sep 15;4:e08649 PubMed

Curr Biol. 2005 Dec 6;15(23):2156-63 PubMed

Mol Biol Cell. 2008 Jan;19(1):207-15 PubMed

J Cell Biol. 2016 Jun 20;213(6):679-92 PubMed

Dev Cell. 2014 Aug 11;30(3):343-52 PubMed

Mol Biol Cell. 2001 Jul;12(7):2047-60 PubMed

J Cell Biol. 1999 Aug 9;146(3):585-96 PubMed

Mol Biol Cell. 2011 Nov;22(22):4302-11 PubMed

Proc Natl Acad Sci U S A. 2016 Aug 2;113(31):8729-34 PubMed

Nat Cell Biol. 2015 Jul;17(7):907-16 PubMed

Elife. 2014 Apr 30;3:e02208 PubMed

Nat Cell Biol. 2000 Jun;2(6):365-70 PubMed

Hum Mol Genet. 2014 Nov 15;23(22):5961-75 PubMed

Trends Cell Biol. 2015 May;25(5):296-307 PubMed

Curr Top Dev Biol. 2000;49:55-73 PubMed

Curr Biol. 2015 Jan 19;25(2):131-40 PubMed

Nat Rev Mol Cell Biol. 2011 Oct 12;12(11):709-21 PubMed

Front Plant Sci. 2013 Nov 27;4:480 PubMed

J Cell Sci. 2002 Jun 1;115(Pt 11):2423-31 PubMed

Plant Cell. 2012 Mar;24(3):1158-70 PubMed

Mol Biol Cell. 2010 Nov 15;21(22):3963-72 PubMed

Cell Cycle. 2015;14(6):902-19 PubMed

Plant Cell Physiol. 2014 Sep;55(9):1636-45 PubMed

Mol Biol Cell. 2013 Nov;24(21):3337-49 PubMed

Nat Cell Biol. 2006 Feb;8(2):137-47 PubMed

Nat Commun. 2014;5:3270 PubMed

Mol Biol Cell. 2002 Sep;13(9):3235-45 PubMed

EMBO J. 2010 Jan 6;29(1):120-30 PubMed

Plant J. 1998 Dec;16(6):697-707 PubMed

J Biol Chem. 2010 Jul 16;285(29):22658-65 PubMed

Nat Commun. 2016 Jul 13;7:12187 PubMed

Trends Cell Biol. 2016 Feb;26(2):80-87 PubMed

Nat Rev Mol Cell Biol. 2015 Dec;16(12):711-26 PubMed

Curr Biol. 2014 Nov 17;24(22):2708-13 PubMed

Nat Cell Biol. 2015 Sep;17(9):1089-93 PubMed

Nat Cell Biol. 2010 Feb;12(2):164-9 PubMed

J Biol Chem. 2016 Oct 28;291(44):23112-23125 PubMed

Curr Biol. 2004 Oct 26;14(20):1801-11 PubMed

Nat Cell Biol. 2014 Sep;16(9):852-63 PubMed

J Cell Physiol. 2012 Jan;227(1):367-82 PubMed

EMBO J. 1997 Dec 1;16(23):6985-95 PubMed

Plant Cell. 2012 Mar;24(3):1171-87 PubMed

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