An Assay of Selected Serum Amino Acids in Patients with Type 2 Diabetes Mellitus
Jazyk angličtina Země Polsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26467133
DOI
10.17219/acem/29223
Knihovny.cz E-zdroje
- MeSH
- aminokyseliny krev MeSH
- biologické markery krev MeSH
- chromatografie iontoměničová MeSH
- diabetes mellitus 2. typu krev diagnóza MeSH
- fluorescenční spektrometrie MeSH
- glykovaný hemoglobin analýza MeSH
- krevní glukóza analýza MeSH
- laboratorní automatizace MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- studie případů a kontrol MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- aminokyseliny MeSH
- biologické markery MeSH
- glykovaný hemoglobin MeSH
- hemoglobin A1c protein, human MeSH Prohlížeč
- krevní glukóza MeSH
BACKGROUND: Amino acids are the building blocks of proteins. In case of insulin resistance, which is typical for type 2 diabetes mellitus (T2DM), proteolysis is increased and protein synthesis is decreased; therefore, we can observe changes in the levels of amino acids in diabetics vs. non-diabetics. OBJECTIVES: The aim of this study was to find differences in the levels of selected amino acids between patients with diabetes (type 2) and a control group. MATERIAL AND METHODS: Amino acids were derivatized with naphthalene-2,3-dicarboxaldehyde in the presence of potassium cyanide to form fluorescent 1-cyanobenz(f)isoindole product. Amino acids derivatives were measured using a high-performance liquid chromatography with fluorescence detection. The serum levels of glucose were determined using an automatic biochemistry analyzer, glycated hemoglobin HbA1c was measured by cation exchange chromatography. RESULTS: A total of 19 serum amino acids in T2DM patients and non-diabetics were measured. There were 9 amino acids, which were significantly different in these groups (p<0.05). Significantly decreased levels of arginine, asparagine, glycine, serine, threonine and significantly increased levels of alanine, isoleucine, leucine, valine in diabetics were found. CONCLUSIONS: Significant difference in metabolism of amino acids between diabetics and non-diabetics were observed. The altered levels of amino acids in diabetic patients could be a suitable predictor of diabetes.
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