An Assay of Selected Serum Amino Acids in Patients with Type 2 Diabetes Mellitus
Language English Country Poland Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26467133
DOI
10.17219/acem/29223
Knihovny.cz E-resources
- MeSH
- Amino Acids blood MeSH
- Biomarkers blood MeSH
- Chromatography, Ion Exchange MeSH
- Diabetes Mellitus, Type 2 blood diagnosis MeSH
- Spectrometry, Fluorescence MeSH
- Glycated Hemoglobin analysis MeSH
- Blood Glucose analysis MeSH
- Automation, Laboratory MeSH
- Middle Aged MeSH
- Humans MeSH
- Aged MeSH
- Case-Control Studies MeSH
- Chromatography, High Pressure Liquid MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amino Acids MeSH
- Biomarkers MeSH
- Glycated Hemoglobin A MeSH
- hemoglobin A1c protein, human MeSH Browser
- Blood Glucose MeSH
BACKGROUND: Amino acids are the building blocks of proteins. In case of insulin resistance, which is typical for type 2 diabetes mellitus (T2DM), proteolysis is increased and protein synthesis is decreased; therefore, we can observe changes in the levels of amino acids in diabetics vs. non-diabetics. OBJECTIVES: The aim of this study was to find differences in the levels of selected amino acids between patients with diabetes (type 2) and a control group. MATERIAL AND METHODS: Amino acids were derivatized with naphthalene-2,3-dicarboxaldehyde in the presence of potassium cyanide to form fluorescent 1-cyanobenz(f)isoindole product. Amino acids derivatives were measured using a high-performance liquid chromatography with fluorescence detection. The serum levels of glucose were determined using an automatic biochemistry analyzer, glycated hemoglobin HbA1c was measured by cation exchange chromatography. RESULTS: A total of 19 serum amino acids in T2DM patients and non-diabetics were measured. There were 9 amino acids, which were significantly different in these groups (p<0.05). Significantly decreased levels of arginine, asparagine, glycine, serine, threonine and significantly increased levels of alanine, isoleucine, leucine, valine in diabetics were found. CONCLUSIONS: Significant difference in metabolism of amino acids between diabetics and non-diabetics were observed. The altered levels of amino acids in diabetic patients could be a suitable predictor of diabetes.
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