Microtubule-crosslinking motor proteins, which slide antiparallel microtubules, are required for the remodeling of microtubule networks. Hitherto, all microtubule-crosslinking motors have been shown to slide microtubules at a constant velocity until no overlap remains between them, leading to the breakdown of the initial microtubule geometry. Here, we show in vitro that the sliding velocity of microtubules, driven by human kinesin-14 HSET, decreases when microtubules start to slide apart, resulting in the maintenance of finite-length microtubule overlaps. We quantitatively explain this feedback using the local interaction kinetics of HSET with overlapping microtubules that cause retention of HSET in shortening overlaps. Consequently, the increased HSET density in the overlaps leads to a density-dependent decrease in sliding velocity and the generation of an entropic force that antagonizes the force exerted by the motors. Our results demonstrate that a spatial arrangement of microtubules can regulate the collective action of molecular motors through the local alteration of their individual interaction kinetics.

AMOLF Amsterdam the Netherlands

B CUBE Center for Molecular Bioengineering Technische Universität Dresden Dresden Germany

Institute of Biotechnology CAS BIOCEV Vestec Czech Republic

Max Planck Institute of Molecular Cell Biology and Genetics Dresden Germany

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Electro-detachment of kinesin motor domain from microtubule in silico

Anillin propels myosin-independent constriction of actin rings

Diffusive tail anchorage determines velocity and force produced by kinesin-14 between crosslinked microtubules