Constriction of the cytokinetic ring, a circular structure of actin filaments, is an essential step during cell division. Mechanical forces driving the constriction are attributed to myosin motor proteins, which slide actin filaments along each other. However, in multiple organisms, ring constriction has been reported to be myosin independent. How actin rings constrict in the absence of motor activity remains unclear. Here, we demonstrate that anillin, a non-motor actin crosslinker, indispensable during cytokinesis, autonomously propels the contractility of actin bundles. Anillin generates contractile forces of tens of pico-Newtons to maximise the lengths of overlaps between bundled actin filaments. The contractility is enhanced by actin disassembly. When multiple actin filaments are arranged into a ring, this contractility leads to ring constriction. Our results indicate that passive actin crosslinkers can substitute for the activity of molecular motors to generate contractile forces in a variety of actin networks, including the cytokinetic ring.

B CUBE Center for Molecular Bioengineering TU Dresden Dresden Germany

Cluster of Excellence Physics of Life Technische Universität Dresden Dresden Germany

CytoMorpho Lab Laboratoire Physiologie Cellulaire and Végétale Institut de recherche interdisciplinaire de Grenoble Commissariat à l'énergie atomique et aux énergies alternatives Grenoble France

Institute of Biotechnology Czech Academy of Sciences BIOCEV Vestec Prague West Czechia

Max Planck Institute of Molecular Cell Biology and Genetics Dresden Germany

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CKAP5 enables formation of persistent actin bundles templated by dynamically instable microtubules

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