Mid-exponential cultures of two traditional biotechnological yeast species, winery Saccharomyces cerevisiae and the less ethanol tolerant bottom-fermenting brewery Saccharomyces pastorianus, were exposed to different concentrations of added ethanol (3, 5 and 8%) The degree of ethanol-induced cell stress was assessed by measuring the cellular activity of superoxide dismutase (SOD), level of lipid peroxidation products, changes in cell lipid content and fatty acid profile. The resveratrol as an antioxidant was found to decrease the ethanol-induced rise of SOD activity and suppress the ethanol-induced decrease in cell lipids. A lower resveratrol concentration (0.5 mg/l) even reduced the extent of lipid peroxidation in cells. Resveratrol also alleviated ethanol-induced changes in cell lipid composition in both species by strongly enhancing the proportion of saturated fatty acids and contributing thereby to membrane stabilization. Lower resveratrol concentrations could thus diminish the negative effects of ethanol stress on yeast cells and improve their physiological state. These effects may be utilized to enhance yeast vitality in high-ethanol-producing fermentations or to increase the number of yeast generations in brewery.

Department of Biotechnology University of Chemistry and Technology Prague Technická 5 166 28 Prague 6 Dejvice Czech Republic

Institute of Microbiology CAS Vídeňská 1083 142 20 Prague Czech Republic

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Food Chem Toxicol. 2014 May;67:72-9 PubMed

Nutr Metab Cardiovasc Dis. 2011 Jan;21(1):46-53 PubMed

Ann N Y Acad Sci. 2003 Dec;1010:365-73 PubMed

Lett Appl Microbiol. 2017 Feb;64(2):103-110 PubMed

Life Sci. 2007 Feb 20;80(11):1033-9 PubMed

Appl Microbiol Biotechnol. 2009 Nov;85(2):253-63 PubMed

Lett Appl Microbiol. 1997 Oct;25(4):249-53 PubMed

Appl Environ Microbiol. 2014 May;80(10):2966-72 PubMed

Microbiol Res. 2014 Dec;169(12):907-14 PubMed

Crit Rev Microbiol. 1986;13(3):219-80 PubMed

Dtsch Tierarztl Wochenschr. 2007 Sep;114(9):345-8 PubMed

J Agric Food Chem. 2005 May 18;53(10):4182-6 PubMed

Appl Microbiol Biotechnol. 2010 Jul;87(3):829-45 PubMed

Biotechnol Bioeng. 1988 Jul 20;32(3):374-8 PubMed

J Biosci Bioeng. 2013 Dec;116(6):697-705 PubMed

Folia Microbiol (Praha). 1999;44(6):587-624 PubMed

J Appl Microbiol. 2010 Jul;109(1):13-24 PubMed

Biochem Pharmacol. 2009 Mar 15;77(6):1053-63 PubMed

J Ind Microbiol Biotechnol. 2014 May;41(5):869-78 PubMed

Appl Environ Microbiol. 2003 Mar;69(3):1499-503 PubMed

Int J Food Microbiol. 2013 Feb 15;161(3):209-13 PubMed

Can J Biochem Physiol. 1959 Aug;37(8):911-7 PubMed

J Biol Chem. 2000 Sep 1;275(35):27393-8 PubMed

J Med Food. 2008 Mar;11(1):55-61 PubMed

FEBS Lett. 2007 Jun 26;581(16):2935-42 PubMed

Arch Toxicol. 2015 Oct;89(10):1681-93 PubMed

Biochim Biophys Acta. 1989 Jan 23;1001(1):102-6 PubMed

Biometals. 2007 Feb;20(1):27-36 PubMed

Anal Biochem. 1976 May 7;72:248-54 PubMed

Life Sci. 2000 Jan 14;66(8):663-73 PubMed

Environ Toxicol Pharmacol. 2002 Jul;11(3-4):321-33 PubMed

FEMS Yeast Res. 2013 Dec;13(8):804-19 PubMed

Int J Food Microbiol. 2011 Feb 28;145(2-3):426-31 PubMed

Front Oncol. 2012 Jun 20;2:64 PubMed

Yeast. 2012 Jul;29(7):251-63 PubMed

FEBS Lett. 2002 Jun 19;521(1-3):47-52 PubMed

Biochem Biophys Res Commun. 2005 Apr 29;330(1):271-8 PubMed

Folia Microbiol (Praha). 1995;40(2):131-52 PubMed

FEMS Microbiol Lett. 2006 Feb;255(2):308-15 PubMed

Folia Microbiol (Praha). 1995;40(5):508-10 PubMed