The aim of the project was to determine any effect of hyperbaric air on Bacteroides fragilis strains cultivated under hyperbaric conditions. Previously, it was hypothesized that there was a correlation between the presence of Bacteroides bacteria in patients preferring a meaty diet and cancer of the small intestine, and particularly of the large intestine and rectum. With respect to the fact that Bacteroides fragilis (BAFR) group are important producers of endotoxins, measurement and statistical evaluation of endotoxin production by individual strains of isolated Bacteroides species were used to compare bacteria isolated from various clinical samples from patients with colon and rectum cancer in comparison with strains isolated from other non-cancer diagnoses. Endotoxin production was proven by quantitative detection using the limulus amebocyte lysate (LAL) test in EU/mL. Production of endotoxins in these bacteria cultured under hyperbaric air conditions was higher than those strains cultured under normobaric anaerobic conditions. But these differences in endotoxin production were not statistically significant (t test with log-transformed data, p value = 0.0910). Based on a two-tier t test for lognormal data, it is possible to cautiously conclude that a statistically significant difference was found between endotoxin production by Bacteroides fragilis strains isolated from non-carcinoma diagnoses (strains (1-6) and strains isolated from colorectal carcinoma diagnoses (strains 7-8; Wilcoxon non-parametric test p = 0.0132; t test = 0.1110; t test with log-transformed data, p value = 0.0294).

Centre of Hyperbaric Medicine Ostrava City Hospital Ostrava Czech Republic

Czech Anaerobic Bacteria Reference Laboratory Faculty of Medicine University of Ostrava Ostrava Zabreh Czech Republic

Department of Biomedical Sciences Institute of Microbiology and Immunology Faculty of Medicine University of Ostrava Syllabova 19 703 00 Ostrava Zabreh Czech Republic

Department of Infection Diagnostic Litomyšl Town Hospital of Pardubice Region Litomyšl Czech Republic

Department of Nonlinear Modelling Institute of Computer Science Academy of Science of the Czech Republic Prague Czech Republic

Faculty of Public Health Slovak Medical University Bratislava Slovak Republic

National Institute of Public Health Prague Czech Republic

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