Saliva of Ixodes ricinus enhances TBE virus replication in dendritic cells by modulation of pro-survival Akt pathway
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
29156399
DOI
10.1016/j.virol.2017.11.008
PII: S0042-6822(17)30379-3
Knihovny.cz E-zdroje
- Klíčová slova
- Dendritic cells, Signaling, TBEV, Tick saliva,
- MeSH
- apoptóza MeSH
- arachnida jako vektory virologie MeSH
- dendritické buňky cytologie metabolismus virologie MeSH
- klíště virologie MeSH
- klíšťová encefalitida metabolismus patofyziologie přenos virologie MeSH
- lidé MeSH
- morčata MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- protoonkogenní proteiny c-akt genetika metabolismus MeSH
- replikace viru MeSH
- sliny virologie MeSH
- transkripční faktor STAT1 genetika metabolismus MeSH
- viry klíšťové encefalitidy genetika fyziologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- morčata MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- protoonkogenní proteiny c-akt MeSH
- transkripční faktor STAT1 MeSH
It has been suggested that tick saliva facilitates transmission of tick-borne encephalitis virus (TBEV) to vertebrates. The mechanism of this facilitation has not been elucidated yet. Since dendritic cells (DCs) are among first cells attacked by the virus, we examined the amount of virus and changes induced by saliva in TBEV-infected DCs. We found that virus replication was significantly increased by saliva of Ixodes ricinus tick. Next, saliva-induced enhancement of Akt pathway activation was observed in TBEV-infected DCs. Akt mediated pathway is known for its anti-apoptotic and pro-survival effects. Accordingly, apoptosis of TBEV-infected DCs was declined and cellular viability increased in the presence of tick saliva. Saliva-induced enhancement of STAT1 and NF-κB was also observed in TBEV-infected DCs. In conclusion, we suggest that tick saliva provides pro-survival and anti-apoptotic signals to infected DCs via upregulation of Akt, which may have positive consequences for TBEV replication and transmission.
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