Analysis of Siamese Crocodile (Crocodylus siamensis) Eggshell Proteome
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
15-01948S
Grantová Agentura České Republiky - International
16-02972S
Grantová Agentura České Republiky - International
PubMed
29168058
DOI
10.1007/s10930-017-9750-x
PII: 10.1007/s10930-017-9750-x
Knihovny.cz E-resources
- Keywords
- Crocodile, Eggshell pigment, Proteins of eggshell,
- MeSH
- Alligators and Crocodiles metabolism MeSH
- Reptilian Proteins metabolism MeSH
- Proteome metabolism MeSH
- Egg Shell metabolism MeSH
- Egg Proteins metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- chorion proteins MeSH Browser
- Reptilian Proteins MeSH
- Proteome MeSH
- Egg Proteins MeSH
The proteins and pigment of the eggshell of the Siamese crocodile (Crocodylus siamensis) were analysed. For proteomic analysis, various decalcification methods were used when the two main surface layers were analyzed. These layers are important for antimicrobial defense of egg (particularly the cuticle). We found 58 proteins in both layers, of which 4 were specific for the cuticle and 26 for the palisade (honeycomb) layer. Substantial differences between proteins in the eggshell of crocodile and previously described birds' eggshells exist (both in terms of quality and quantity), however, the entire proteome of Crocodilians has not been described yet. The most abundant protein was thyroglobulin. The role of determined proteins in the eggshell of the Siamese crocodile is discussed. For the first time, the presence of porphyrin pigment is reported in a crocodilian eggshell, albeit in a small amount (about 2 to 3 orders of magnitude lower than white avian eggs).
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