Molecular identification of Leishmania spp. isolates causes cutaneous leishmaniasis (CL) in Sanliurfa Province, Turkey, where CL is highly endemic
Language English Country United States Media print-electronic
Document type Journal Article
Grant support
FED.13.16
Gaziantep University Scientific Research Fund
PubMed
29214528
DOI
10.1007/s12223-017-0556-1
PII: 10.1007/s12223-017-0556-1
Knihovny.cz E-resources
- MeSH
- Child MeSH
- Adult MeSH
- Infant MeSH
- Skin microbiology pathology MeSH
- Leishmania classification genetics MeSH
- Leishmaniasis, Cutaneous diagnosis epidemiology microbiology pathology MeSH
- Middle Aged MeSH
- Humans MeSH
- DNA, Ribosomal Spacer genetics MeSH
- Adolescent MeSH
- Young Adult MeSH
- Polymerase Chain Reaction MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Child, Preschool MeSH
- DNA, Protozoan genetics MeSH
- Aged MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Child, Preschool MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Turkey epidemiology MeSH
- Names of Substances
- DNA, Ribosomal Spacer MeSH
- DNA, Protozoan MeSH
Cutaneous leishmaniasis (CL) is an important public health problem in Turkey. CL has been most frequently seen in Sanliurfa. There is an expectation of increase in the population of leishmaniasis cases with the influence of Syrian refugees arriving in Turkey. In this study we aimed to diagnosis of CL and identifying of parasite from Leishmania isolates by using ITS 1 PCR RFLP. Samples were collected from 135 CL patients in Sanliurfa. After the specimens were inoculated in medium NNN, the ones which were cultures positive were cultivated in RPMI 1640 followed by PCR-RFLP. Genomic DNA was extracted phenol-chloroform procedure. Samples were examined by using ITS 1 PCR followed by RFLP analysis. Our results indicated that two species, L. tropica (132 samples) and L. major (3 samples), are responsible for cutaneous leishmaniasis in Sanlıurfa. Our study is the first scientific study in which it is reported molecular analyses of cutaneous leishmaniasis cases caused by L. major in Sanliurfa in Southestern Anatolia Region. Because CL cases caused by L.major are detected in our study, it is considered that genotyping is important for diagnosis of Leishmania and following change of epidemiology.
Department of Biology Gaziantep University Gaziantep Turkey
Department of Clinical Microbiology Harran University Sanliurfa Turkey
Vocational School of Health Service Harran University Sanliurfa Turkey
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