Molecular identification of Leishmania spp. isolates causes cutaneous leishmaniasis (CL) in Sanliurfa Province, Turkey, where CL is highly endemic
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
FED.13.16
Gaziantep University Scientific Research Fund
PubMed
29214528
DOI
10.1007/s12223-017-0556-1
PII: 10.1007/s12223-017-0556-1
Knihovny.cz E-zdroje
- MeSH
- dítě MeSH
- dospělí MeSH
- kojenec MeSH
- kůže mikrobiologie patologie MeSH
- Leishmania klasifikace genetika MeSH
- leishmanióza kožní diagnóza epidemiologie mikrobiologie patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mezerníky ribozomální DNA genetika MeSH
- mladiství MeSH
- mladý dospělý MeSH
- polymerázová řetězová reakce MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- předškolní dítě MeSH
- protozoální DNA genetika MeSH
- senioři MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- kojenec MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Turecko epidemiologie MeSH
- Názvy látek
- mezerníky ribozomální DNA MeSH
- protozoální DNA MeSH
Cutaneous leishmaniasis (CL) is an important public health problem in Turkey. CL has been most frequently seen in Sanliurfa. There is an expectation of increase in the population of leishmaniasis cases with the influence of Syrian refugees arriving in Turkey. In this study we aimed to diagnosis of CL and identifying of parasite from Leishmania isolates by using ITS 1 PCR RFLP. Samples were collected from 135 CL patients in Sanliurfa. After the specimens were inoculated in medium NNN, the ones which were cultures positive were cultivated in RPMI 1640 followed by PCR-RFLP. Genomic DNA was extracted phenol-chloroform procedure. Samples were examined by using ITS 1 PCR followed by RFLP analysis. Our results indicated that two species, L. tropica (132 samples) and L. major (3 samples), are responsible for cutaneous leishmaniasis in Sanlıurfa. Our study is the first scientific study in which it is reported molecular analyses of cutaneous leishmaniasis cases caused by L. major in Sanliurfa in Southestern Anatolia Region. Because CL cases caused by L.major are detected in our study, it is considered that genotyping is important for diagnosis of Leishmania and following change of epidemiology.
Department of Biology Gaziantep University Gaziantep Turkey
Department of Clinical Microbiology Harran University Sanliurfa Turkey
Vocational School of Health Service Harran University Sanliurfa Turkey
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