After its computational inference from human stool metagenomes, the CrAssphage has proven to be the most prevalent phage in the human gut, with presumably very wide geographic distribution. The currently available molecular assays do not sufficiently reflect the CrAssphage sequence variability. Here, we report a novel real-time PCR assay whose primers and probes are derived from data of multiple CrAssphage strains obtained from gut viral metagenomes of European, Asian, and African subjects. This assay can be useful in analyses of putative bacterial host co-occurence, and in association studies of non-infectious diseases where the phage may modify the content of gut bacteriomes.

2nd Faculty of Medicine Department of Pediatrics Charles University Prague and University Hospital Motol Prague Czech Republic

Azerbaijan Medical University Baku Azerbaijan

Centre of Epidemiology and Microbiology National Institute of Public Health Prague Czech Republic

Department of Pediatrics Ebonyi State University Abakaliki Nigeria

Department of Pediatrics School of Medicine University of Jordan Amman Jordan

Faculty of Medicine and Life Sciences Department of Virology University of Tampere Tampere Finland

Faculty of Medicine Department of Paediatrics and Child Health University of Khartoum Khartoum Sudan

Fimlab Laboratories Pirkanmaa Hospital District Tampere Finland

Sudan Childhood Diabetes Center Khartoum Sudan

Citace poskytuje Crossref.org

Global phylogeography and ancient evolution of the widespread human gut virus crAssphage