The role of non-coding RNAs in different biological processes and diseases is continuously expanding. Next-generation sequencing together with the parallel improvement of bioinformatics analyses allows the accurate detection and quantification of an increasing number of RNA species. With the aim of exploring new potential biomarkers for disease classification, a clear overview of the expression levels of common/unique small RNA species among different biospecimens is necessary. However, except for miRNAs in plasma, there are no substantial indications about the pattern of expression of various small RNAs in multiple specimens among healthy humans. By analysing small RNA-sequencing data from 243 samples, we have identified and compared the most abundantly and uniformly expressed miRNAs and non-miRNA species of comparable size with the library preparation in four different specimens (plasma exosomes, stool, urine, and cervical scrapes). Eleven miRNAs were commonly detected among all different specimens while 231 miRNAs were globally unique across them. Classification analysis using these miRNAs provided an accuracy of 99.6% to recognize the sample types. piRNAs and tRNAs were the most represented non-miRNA small RNAs detected in all specimen types that were analysed, particularly in urine samples. With the present data, the most uniformly expressed small RNAs in each sample type were also identified. A signature of small RNAs for each specimen could represent a reference gene set in validation studies by RT-qPCR. Overall, the data reported hereby provide an insight of the constitution of the human miRNome and of other small non-coding RNAs in various specimens of healthy individuals.

Center for Cancer Epidemiology and Prevention AO City of Health and Science Turin Italy

Department of Clinical and Biological Sciences University of Turin Turin Italy

Department of Colorectal Surgery Clinica S Rita Vercelli Italy

Department of Computer Science University of Turin Turin Italy

Department of Medical and Surgical Sciences University of Catanzaro Catanzaro Italy

Department of Medical Sciences University of Turin Turin Italy

Department of Molecular Biology of Cancer Institute of Experimental Medicine Prague Czech Republic

Department of Surgical Sciences University of Turin and Città della Salute e della Scienza Turin Italy

Italian Institute for Genomic Medicine IIGM Turin Italy

Molecular Biotechnology Center Department of Biotechnology and Health Sciences University of Turin Turin Italy

MRC HPA Centre for Environment and Health School of Public Health Imperial College London London United Kingdom

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