Detection of Distinct Changes in Gene-expression Profiles in Specimens of Tumors and Transition Zones of Tenascin-positive/-negative Head and Neck Squamous Cell Carcinoma
Language English Country Greece Media print
Document type Journal Article
PubMed
29491051
DOI
10.21873/anticanres.12350
PII: 38/3/1279
Knihovny.cz E-resources
- Keywords
- Extracellular matrix *, fibronectin *, galectin *, stroma *, tenascin *, tumor *,
- MeSH
- Fibronectins genetics metabolism MeSH
- Galectin 1 genetics metabolism MeSH
- Gene Ontology MeSH
- Humans MeSH
- Biomarkers, Tumor genetics MeSH
- Head and Neck Neoplasms genetics metabolism surgery MeSH
- Disease-Free Survival MeSH
- Gene Expression Regulation, Neoplastic * MeSH
- Margins of Excision MeSH
- Mucous Membrane metabolism MeSH
- Carcinoma, Squamous Cell genetics metabolism surgery MeSH
- Gene Expression Profiling methods MeSH
- Tenascin genetics metabolism MeSH
- Transcriptome * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Fibronectins MeSH
- Galectin 1 MeSH
- Biomarkers, Tumor MeSH
- Tenascin MeSH
BACKGROUND/AIM: Having previously initiated genome-wide expression profiling in head and neck squamous cell carcinoma (HNSCC) for regions of the tumor, the margin of surgical resecate (MSR) and normal mucosa (NM), we here proceed with respective analysis of cases after stratification according to the expression status of tenascin (Ten). MATERIALS AND METHODS: Tissue specimens of each anatomical site were analyzed by immunofluorescent detection of Ten, fibronectin (Fn) and galectin-1 (Gal-1) as well as by microarrays. RESULTS: Histopathological examination demonstrated that Ten+Fn+Gal-1+ co-expression occurs more frequently in samples of HNSCC (55%) than in NM (9%; p<0.01). Contrary, the Ten-Fn+Gal-1- (45%) and Ten-Fn-Gal-1- (39%) status occurred with significantly (p<0.01) higher frequency than in HNSCC (3% and 4%, respectively). In MSRs, different immunophenotypes were distributed rather equally (Ten+Fn+Gal-1+=24%; Ten-Fn+Gal-1-=36%; Ten-Fn-Gal-1-=33%), differing to the results in tumors (p<0.05). Absence/presence of Ten was used for stratification of patients into cohorts without a difference in prognosis, to comparatively examine gene-activity signatures. Microarray analysis revealed i) expression of several tumor progression-associated genes in Ten+ HNSCC tumors and ii) a strong up-regulation of gene expression assigned to lipid metabolism in MSRs of Ten- tumors, while NM profiles remained similar. CONCLUSION: The presented data reveal marked and specific changes in tumors and MSR specimens of HNSCC without a separation based on prognosis.
BIOCEV Charles University 1st Faculty of Medicine Charles University Vestec Czech Republic
Institute of Anatomy 1st Faculty of Medicine Charles University Prague Czech Republic
References provided by Crossref.org
The Head and Neck Squamous Cell Carcinoma Microenvironment as a Potential Target for Cancer Therapy
