BACKGROUND/AIM: Having previously initiated genome-wide expression profiling in head and neck squamous cell carcinoma (HNSCC) for regions of the tumor, the margin of surgical resecate (MSR) and normal mucosa (NM), we here proceed with respective analysis of cases after stratification according to the expression status of tenascin (Ten). MATERIALS AND METHODS: Tissue specimens of each anatomical site were analyzed by immunofluorescent detection of Ten, fibronectin (Fn) and galectin-1 (Gal-1) as well as by microarrays. RESULTS: Histopathological examination demonstrated that Ten+Fn+Gal-1+ co-expression occurs more frequently in samples of HNSCC (55%) than in NM (9%; p<0.01). Contrary, the Ten-Fn+Gal-1- (45%) and Ten-Fn-Gal-1- (39%) status occurred with significantly (p<0.01) higher frequency than in HNSCC (3% and 4%, respectively). In MSRs, different immunophenotypes were distributed rather equally (Ten+Fn+Gal-1+=24%; Ten-Fn+Gal-1-=36%; Ten-Fn-Gal-1-=33%), differing to the results in tumors (p<0.05). Absence/presence of Ten was used for stratification of patients into cohorts without a difference in prognosis, to comparatively examine gene-activity signatures. Microarray analysis revealed i) expression of several tumor progression-associated genes in Ten+ HNSCC tumors and ii) a strong up-regulation of gene expression assigned to lipid metabolism in MSRs of Ten- tumors, while NM profiles remained similar. CONCLUSION: The presented data reveal marked and specific changes in tumors and MSR specimens of HNSCC without a separation based on prognosis.

BIOCEV Charles University 1st Faculty of Medicine Charles University Vestec Czech Republic

Department of Biomedical Research East Slovak Institute of Cardiovascular Diseases Inc Kosice Slovak Republic

Department of Otorhinolaryngology and Head and Neck Surgery 3rd Faculty of Medicine University Hospital Královské Vinohrady Charles University Prague Prague Czech Republic

Department of Otorhinolaryngology Head and Neck Surgery 1st Faculty of Medicine Charles University and University Hospital Motol Prague Czech Republic

Department of Pharmacology Faculty of Medicine Pavol Jozef Šafárik University in Košice Kosice Slovak Republic

Institute of Anatomy 1st Faculty of Medicine Charles University Prague Czech Republic

Institute of Physiological Chemistry Faculty of Veterinary Medicine Ludwig Maximilians University Munich Germany

Laboratory of Genomics and Bioinformatics Institute of Molecular Genetics Academy of Sciences of the Czech Republic Prague Czech Republic

Citace poskytuje Crossref.org

Cancer-Associated Fibroblasts Influence the Biological Properties of Malignant Tumours via Paracrine Secretion and Exosome Production

The Head and Neck Squamous Cell Carcinoma Microenvironment as a Potential Target for Cancer Therapy