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Záznam pochází z PubMed

Cholesterol Protects the Oxidized Lipid Bilayer from Water Injury: An All-Atom Molecular Dynamics Study

Owen, Michael C
Autor Owen, Michael C Institute of Complex Systems: Structural Biochemistry (ICS-6), Forschungszentrum Jülich, 52425, Jülich, Germany. michael.owen@ceitec.muni.cz CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic. michael.owen@ceitec.muni.cz
Kulig, Waldemar
Autor Kulig, Waldemar ORCID Department of Physics, University of Helsinki, P. O. Box 64, 00014, Helsinki, Finland Department of Physics, Tampere University of Technology, P. O. Box 692, 33101, Tampere, Finland
Rog, Tomasz
Autor Rog, Tomasz ORCID Department of Physics, University of Helsinki, P. O. Box 64, 00014, Helsinki, Finland Department of Physics, Tampere University of Technology, P. O. Box 692, 33101, Tampere, Finland
Vattulainen, Ilpo
Autor Vattulainen, Ilpo ORCID Department of Physics, University of Helsinki, P. O. Box 64, 00014, Helsinki, Finland Department of Physics, Tampere University of Technology, P. O. Box 692, 33101, Tampere, Finland MEMPHYS - Center for Biomembrane Physics, University of Southern Denmark, Odense, Denmark
Strodel, Birgit
Autor Strodel, Birgit ORCID Institute of Complex Systems: Structural Biochemistry (ICS-6), Forschungszentrum Jülich, 52425, Jülich, Germany. b.strodel@fz-juelich.de Institute of Theoretical and Computational Chemistry, Heinrich Heine University Düsseldorf, Universitätsstrasse 1, 40225, Düsseldorf, Germany. b.strodel@fz-juelich.de

The Journal of membrane biology. 2018 Jun ; 251 (3) : 521-534. [epub] 20180317

J Membr Biol
ISSN 1432-1424 | 0022-2631
Zdroj

Jazyk angličtina Země Spojené státy americké Médium print-electronic

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz https://www.medvik.cz/link/pmid29550877

Grantová podpora
263410 FiDiPro - International
290974 European Research Council - International
SFB 1208 Deutsche Forschungsgemeinschaft - International

PubMed 29550877
DOI 10.1007/s00232-018-0028-9
PII: 10.1007/s00232-018-0028-9
Knihovny.cz E-zdroje

Klíčová slova
Cholesterol protection, Lipid oxidation, Oxidative stress, Oxidized membranes, Pore formation,
MeSH
cholesterol chemie metabolismus MeSH
fosfatidylcholiny chemie metabolismus MeSH
fosfolipidy chemie metabolismus MeSH
fosforylcholin analogy a deriváty chemie metabolismus MeSH
lipidové dvojvrstvy chemie metabolismus MeSH
oxidace-redukce MeSH
oxidační stres MeSH
simulace molekulární dynamiky MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH
Názvy látek
1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine MeSH Prohlížeč
1-palmitoyl-2-oleoylphosphatidylcholine MeSH Prohlížeč
cholesterol MeSH
fosfatidylcholiny MeSH
fosfolipidy MeSH
fosforylcholin MeSH
lipidové dvojvrstvy MeSH

In an effort to delineate how cholesterol protects membrane structure under oxidative stress conditions, we monitored the changes to the structure of lipid bilayers comprising 30 mol% cholesterol and an increasing concentration of Class B oxidized 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) glycerophospholipids, namely, 1-palmitoyl-2-(9'-oxo-nonanoyl)-sn-glycero-3-phosphocholine (PoxnoPC), and 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PazePC), using atomistic molecular dynamics simulations. Increasing the content of oxidized phospholipids (oxPLs) from 0 to 60 mol% oxPL resulted in a characteristic reduction in bilayer thickness and increase in area per lipid, thereby increasing the exposure of the membrane hydrophobic region to water. However, cholesterol was observed to help reduce water injury by moving into the bilayer core and forming more hydrogen bonds with the oxPLs. Cholesterol also resists altering its tilt angle, helping to maintain membrane integrity. Water that enters the 1-nm-thick core region remains part of the bulk water on either side of the bilayer, with relatively few water molecules able to traverse through the bilayer. In cholesterol-rich membranes, the bilayer does not form pores at concentrations of 60 mol% oxPL as was shown in previous simulations in the absence of cholesterol.

CEITEC Central European Institute of Technology Masaryk University Kamenice 753 5 625 00 Brno Czech Republic

Department of Physics Tampere University of Technology P O Box 692 33101 Tampere Finland

Department of Physics University of Helsinki P O Box 64 00014 Helsinki Finland

Institute of Complex Systems Structural Biochemistry Forschungszentrum Jülich 52425 Jülich Germany

Institute of Theoretical and Computational Chemistry Heinrich Heine University Düsseldorf Universitätsstrasse 1 40225 Düsseldorf Germany

MEMPHYS Center for Biomembrane Physics University of Southern Denmark Odense Denmark

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